Team:TU Munich

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  <p style="font-size:188%"><b> Welcome to E.XPRESS3D</b></p>
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                <hr />
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  <p style="font-size:120%"> This year we aim to develop a light-controlled 3D-printer by innovative utilization of optogenetics. As a first step, we want to develop a genetic logical AND-gate sensitive to light of two different wavelengths (e.g. blue and red light). The bacteria are then immobilized in a transparent gel matrix, where they can be precisely actuated when hit by both blue and red light at the same time. If both of these inputs are positive, gene expression is induced. Various different gene products can be expressed using this system.</p>
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<p style="font-size:120%"> If you want to learn more about our project, the modeling, our human practice projects or the team, click on the pictures below. </p>
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<ul>
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<li><a href="https://2011.igem.org/Team:TU_Munich/project/introduction">Introduction</a></li><br>
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<p>We give a short introduction on the topic and explain how we came up with our idea.</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/project/design">Design</a></li><br>
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<p>The functionality of our project is described in a more detailed fashion.</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/project/applications">Applications</a></li><br>
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<p>This is a short outlook on how our AND-Gate could be used in future applications.</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/project/data">Data Page</a></li><br>
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<p>This section gives an overview over the created parts and characterised BioBricks.</p>
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<div class="ca-item-main" style="background-image:url(https://static.igem.org/mediawiki/2011/0/0f/TumLab.jpg);opacity:0.9;">
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<li><a href="https://2011.igem.org/Team:TU_Munich/lab/results">Results</a></li><br>
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<p>Here you can see an overview over our solid matrix experiments and BioBrick characterisations.</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/lab/safety">Safety</a></li><br>
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<p>All possibly arising questions concerning safety are resolved here.</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/lab/notebook">Labbook</a></li><br>
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<p>This is an extensive documentation of our lab work, that also includes our protocols and the lightbox design.</p>
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</ul>
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<br>
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<ul>
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<li><a href="https://2011.igem.org/Team:TU_Munich/model/modeloverview">Overview</a></li><br>
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<p>This overview depicts the different components of our model.</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/model/guide">Guide</a></li><br>
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<p>Here we give an explanation on how we derived the equations for our model.</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/model/combined">Combined Model</a></li><br>
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<p>This shows our modeling results for the complete system.</p>
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<div class="ca-item-main" style="background-image:url(https://static.igem.org/mediawiki/2011/4/42/TumHumanP.jpg);opacity:0.9;">
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<ul>
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<li><a href="https://2011.igem.org/Team:TU_Munich/human/comics">Overview</a></li><br>
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<p>Here you can find a short Introduction to our efforts including our video, comic and book.</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/human/media">Media</a></li><br>
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<p>Here we list where we were featured in the media.</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/human/highschools">High Schools</a></li><br>
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<p>This section shows our experiences with students on a high school level.</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/human/kindergarten">Kindergarten</a></li><br>
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<p>We even visited kids in kindergartens and and gave them an insight into synthetic biology!</p>
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</ul>
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<div class="ca-item ca-item-5">
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<li><a href="https://2011.igem.org/Team:TU_Munich/team/teamoverview">Overview</a></li><br>
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<p>That's us!</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/team/university">TU Munich</a></li><br>
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<p>This is our University.</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/team/lab">Simmel Lab</a></li><br>
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<p>Here you can see our Lab.</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/team/prevteam">2010 Team</a></li><br>
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<p>And this is the previous team.</p>
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<a href="#" class="ca-more">more...</a>
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<a href="#" class="ca-close">close</a>
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<br>
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<ul>
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<li><a href="https://2011.igem.org/Team:TU_Munich/extra/extraoverview">Overview</a></li><br>
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<p>Here you can discover everything else we did during the iGEM competition. Don't forget to have a look at the pictures!</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/extra/attributions">Attributions</a></li><br>
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<p>We show everything that was not done by us.</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/extra/partners">Partners</a></li><br>
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<p>Everybody who helped us is listed here. Big Thanks!</p>
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<li><a href="https://2011.igem.org/Team:TU_Munich/extra/jamboree">Jamboree</a></li><br>
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<p>Find out about our experience at the World Jamboree.</p>
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</ul>
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Homepage at TUM: http://igem.ph.tum.de/
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<div class="subcontent ui-corner-all" id="twitter_div" style="margin-right:20px;float:left;height:250px;width:171px;top:20px;position:relative;overflow:hidden;"><h4>Latest Tweets:</h4><hr /><ul id="twitter_update_list"></ul>
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Safety Proposal: [[Team:TU_Munich/team/safety]]
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            <h4>Quicklinks</h4>
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            <hr \>
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            <ul>
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                <li><a href="http://www.igem.org" target="_blank">iGEM Main Page</a></li>
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                <li><a href="https://2011.igem.org/Team:TU_Munich/project/data" target="_blank">Data Page</a></li>
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                <li><a href="https://2011.igem.org/Team:TU_Munich/extra/attribution" target="_blank">Attributions</a></li>
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                <li><a href="http://igem.ph.tum.de/" target="_blank">German Website</a></li>
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                <li><a href="http://www.youtube.com/user/iGEMTUMUNICH2011" target="_blank">Youtube Channel</a></li>
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                <li><a href="http://twitter.com/#!/iGEM_TUM_2011/" target="_blank">Twitter Account</a></li>
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                <li><a href="http://www.facebook.com/pages/IGEM-TU-M%C3%BCnchen/149746078408629/" target="_blank">Facebook Page</a></li>
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                <li><a href="https://2011.igem.org/Team:TU_Munich/extra/german">German Overview</a> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="https://2011.igem.org/Team:TU_Munich/extra/german"><img src="https://static.igem.org/mediawiki/2011/b/be/200px-Flag_of_Germany.svg.png" style="height:25px;" ></a></li>
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|[[Image:TU_Munich_logo.png|200px|right]]
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'''General Project Outline'''
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This year, we aim to develop a light-controlled 3D-printer by innovative utilization of optogenetics. As a first step, we want to develop a genetic logical AND-gate sensitive to light of two different wavelengths (e.g. blue and red light). The bacteria are then immobilized in a transparent gel matrix, where they can be precisely actuated when hit by both blue and red light at the same time. If both of these inputs are positive, gene expression is induced. Various different gene products can be expressed using this system. For example, a simple colored pigment will allow us to create colored three dimensional objects, while expressing collagen and consecutive biomineralization and generation of hydroxylapatite could be used to create bone.
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'''Optogenetical AND-Gate'''
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In order to be able to adress our immobilized cells in a defined spatiotemporal manner, we require two signals which will be irradiated into the gel block from two different directions. For this purpose, we will introduce an optogenetical AND-Gate into the bacteria. This logical gate is based on amber stop-codon suppression via the non-canonical tRNA supD. A light sensitive promoter induces the expression of a mRNA coding for a T7-polymerase, which can only be translated by ribosomes if the correct amber tRNA is present. The tRNA is expressed by a second light-sensitive promoter. Thus, only cells which receive both signals produce the desired substances.
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'''Light Sensory Domains'''
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We have three candidates which can be used as light sensitive promoters: the promoter of YcgF/E as blue light sensor, Cph8 as red light sensor, and ccaR/ccaS as green-light sensor. At this point, we are inclined to use a combination of the red and blue light sensor systems, because of the great difference between the absorbtion maxima of the light sensory domains. We expect that this assembly will be the one with the lowest amount of unspecific gene expression which could occur due to overlapping of the absorption spectrums of the sensory domains.
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'''Progress'''
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Construct design has been completed while cloning is still in progress. Further more, we found a suitable solid matrix which is, on the one hand, clear enough to be penetrated by light beams without refracting them and, on the other hand, solidifies at a temperature that is low enough to allow for cell immobilization without harming the bacteria.
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!align="center"|[[Team:TU_Munich|Home]]
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!align="center"|[[Team:TU_Munich/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=TU_Munich Official Team Profile]
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!align="center"|[[Team:TU_Munich/Project|Project]]
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!align="center"|[[Team:TU_Munich/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:TU_Munich/Modeling|Modeling]]
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Latest revision as of 01:51, 29 October 2011

Welcome to E.XPRESS3D

This year we aim to develop a light-controlled 3D-printer by innovative utilization of optogenetics. As a first step, we want to develop a genetic logical AND-gate sensitive to light of two different wavelengths (e.g. blue and red light). The bacteria are then immobilized in a transparent gel matrix, where they can be precisely actuated when hit by both blue and red light at the same time. If both of these inputs are positive, gene expression is induced. Various different gene products can be expressed using this system.

If you want to learn more about our project, the modeling, our human practice projects or the team, click on the pictures below.

more...
close
  • Introduction

    • We give a short introduction on the topic and explain how we came up with our idea.

  • Design

    • The functionality of our project is described in a more detailed fashion.

  • Applications

    • This is a short outlook on how our AND-Gate could be used in future applications.

  • Data Page

    • This section gives an overview over the created parts and characterised BioBricks.

more...
close
  • Results

    • Here you can see an overview over our solid matrix experiments and BioBrick characterisations.

  • Safety

    • All possibly arising questions concerning safety are resolved here.

  • Labbook

    • This is an extensive documentation of our lab work, that also includes our protocols and the lightbox design.

more...
close
  • Overview

    • This overview depicts the different components of our model.

  • Guide

    • Here we give an explanation on how we derived the equations for our model.

  • Combined Model

    • This shows our modeling results for the complete system.

more...
close
  • Overview

    • Here you can find a short Introduction to our efforts including our video, comic and book.

  • Media

    • Here we list where we were featured in the media.

  • High Schools

    • This section shows our experiences with students on a high school level.

  • Kindergarten

    • We even visited kids in kindergartens and and gave them an insight into synthetic biology!

more...
close
more...
close
  • Overview

    • Here you can discover everything else we did during the iGEM competition. Don't forget to have a look at the pictures!

  • Attributions

    • We show everything that was not done by us.

  • Partners

    • Everybody who helped us is listed here. Big Thanks!

  • Jamboree

    • Find out about our experience at the World Jamboree.

Latest Tweets:

    Retrieved from "http://2011.igem.org/Team:TU_Munich"