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Team:Paris Liliane Bettencourt/Notebook/2011/10/22/
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Danyel.lee90 (Talk | contribs) (Created page with "==tRNA so far...== ===constructs=== S99: Phyperspank_tRNA_tt S173: Pveg_spoVG_RFP_tt_Phs_tRNA_tt S174: Pveg_spoVG_LacI_tt_Phs_tRNA_tt S105: Pveg_spoVG_T7amber_tt S136: Pveg_spoVG...") |
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==tRNA so far...== | ==tRNA so far...== | ||
===constructs=== | ===constructs=== | ||
| - | S99: Phyperspank_tRNA_tt | + | -S99: Phyperspank_tRNA_tt |
| - | S173: Pveg_spoVG_RFP_tt_Phs_tRNA_tt | + | -S173: Pveg_spoVG_RFP_tt_Phs_tRNA_tt |
| - | S174: Pveg_spoVG_LacI_tt_Phs_tRNA_tt | + | -S174: Pveg_spoVG_LacI_tt_Phs_tRNA_tt |
| - | S105: Pveg_spoVG_T7amber_tt | + | -S105: Pveg_spoVG_T7amber_tt |
| - | S136: Pveg_spoVG_GFPamber_tt | + | -S136: Pveg_spoVG_GFPamber_tt |
| - | S150: double transformation of S99 (tRNA) and S136 (GFPamber) | + | -S150: double transformation of S99 (tRNA) and S136 (GFPamber) |
| - | S177: RECEIVER - pT7_spoVG_T7_spoVG_GFP_t7t_Pveg_spoVG_T7amber_tt | + | -S177: RECEIVER - pT7_spoVG_T7_spoVG_GFP_t7t_Pveg_spoVG_T7amber_tt |
in progress: | in progress: | ||
| - | sequencing of S177 | + | -sequencing of S177 |
| - | sequencing of S174 | + | -sequencing of S174 |
| - | sequencing of LacI-RFP-tRNA | + | -sequencing of LacI-RFP-tRNA |
| - | completing construct: LacI-tRNA + GFPamber | + | -completing construct: LacI-tRNA + GFPamber |
| - | completing construct: pT7_rbs_gfp_t7t + T7amber | + | -completing construct: pT7_rbs_gfp_t7t + T7amber |
===characterization=== | ===characterization=== | ||
Revision as of 03:05, 22 October 2011
tRNA so far...
constructs
-S99: Phyperspank_tRNA_tt -S173: Pveg_spoVG_RFP_tt_Phs_tRNA_tt -S174: Pveg_spoVG_LacI_tt_Phs_tRNA_tt -S105: Pveg_spoVG_T7amber_tt -S136: Pveg_spoVG_GFPamber_tt -S150: double transformation of S99 (tRNA) and S136 (GFPamber) -S177: RECEIVER - pT7_spoVG_T7_spoVG_GFP_t7t_Pveg_spoVG_T7amber_tt
in progress: -sequencing of S177 -sequencing of S174 -sequencing of LacI-RFP-tRNA -completing construct: LacI-tRNA + GFPamber -completing construct: pT7_rbs_gfp_t7t + T7amber
characterization
tRNA + GFPamber with or without IPTG (flouorescence, microscopy, TECAN (to be updated)) T7 autoloop in BL21 with or without IPTG VS. pT7_rbs_gfp_t7t in BL21 with or without IPTG (microscopy (double check), TECAN (to be updated))
To Do: compare basal expression and IPTG induction level of tRNA + GFPamber VS. LacI-tRNA + GFPamber GFP expression with or without IPTG of (LacI)-tRNA with pT7_rbs_gfp_t7t GFP expression with or without IPTG of Receiver + Emitter
