Team:Paris Liliane Bettencourt/Notebook/2011/08/22/
From 2011.igem.org
Contents |
Kevin
Results of PCR Colony
So it seems that 3 clones of tetR:YFP (2.2.1 - 2.2.2 - 2.3.1) and 2 clones of tetO array (4.7.1 -4.8.2) works.
Sent it today to sequencing.
Going to vacation, all instructions are in a paper in the computer room if someone wants to progress in this project !
Axel
ligation of ComS biobricked plasmids
ligation with PSB1C3 (digested in Ecor1 and Pst1) / ComS digested in Ecor1 and Pst1 ligation with K143053 (digested in Spe1 and Pst1) / ComS digested in Xba1 and Pst1
after transformation: get a lot of colonies. Sent to sequencing: it seems that the expected ComS is not ComS
Camille
As we did for the tRNA we decided to synthesize the promoter Hyperspank ba y annealing extension with Klenow polymerase. That polymerase is known for elongating primers at 37°C that way we prevent the primers to detach when the product is heated to reach the elongation temperature of the Taq or phusion.
I launched one control and three tubes.