Team:Paris Liliane Bettencourt/Notebook/2011/08/22/

From 2011.igem.org

(Difference between revisions)
(Kevin)
(Kevin)
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[[File:gelpcrcolonyky.jpg|450px|thumb|center|Gel of PCR Colony - Column 1.2.1 - 1.2.2 - 1.3.1 - 1.3.2 - 2.2.1 - 2.2.2 - 2.3.1 - 2.3.2 - 3.7.1 - 3.7.2 - 3.8.1 - 3.8.2 - 4.7.1 - 4.7.2 - 4.8.1 - 4.8.2]]
[[File:gelpcrcolonyky.jpg|450px|thumb|center|Gel of PCR Colony - Column 1.2.1 - 1.2.2 - 1.3.1 - 1.3.2 - 2.2.1 - 2.2.2 - 2.3.1 - 2.3.2 - 3.7.1 - 3.7.2 - 3.8.1 - 3.8.2 - 4.7.1 - 4.7.2 - 4.8.1 - 4.8.2]]
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So it seems that 3 clones of tetR:YFP (2.2.1 - 2.2.2 - 2.3.1) and 2 clones of tetO array (4.7.1 -4.8.2) works.
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So it seems that 3 clones of tetR:YFP (2.2.1 - 2.2.2 - 2.3.1) and 2 clones of tetO array (4.7.1 -4.8.2) works.<br>
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Sent it today to sequencing.
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Sent it today to sequencing. <br>
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Going to vacation, all instructions are in a paper in the computer room if someone wants to progress in this project !

Revision as of 16:03, 22 August 2011

Kevin

Results of PCR Colony

Gel of PCR Colony - Column 1.2.1 - 1.2.2 - 1.3.1 - 1.3.2 - 2.2.1 - 2.2.2 - 2.3.1 - 2.3.2 - 3.7.1 - 3.7.2 - 3.8.1 - 3.8.2 - 4.7.1 - 4.7.2 - 4.8.1 - 4.8.2

So it seems that 3 clones of tetR:YFP (2.2.1 - 2.2.2 - 2.3.1) and 2 clones of tetO array (4.7.1 -4.8.2) works.
Sent it today to sequencing.

Going to vacation, all instructions are in a paper in the computer room if someone wants to progress in this project !