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Revision as of 16:50, 7 August 2011 (view source) Cyrpaut (Talk | contribs) (→Cyrille) ← Older edit		Revision as of 16:51, 7 August 2011 (view source) Kyauy (Talk | contribs) (→Kevin) Newer edit →
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	== Kevin ==		== Kevin ==
		+
		+	=== Miniprep from glycerol ===
		+	To re-test pDAG464 and pDAG479 plasmids, culture of them overnight has been done and miniprep. Quantification by TECAN :
		+	*pDAG464 : 122,9 ng/uL
		+	*pDAG479 : 204,9ng/uL
		+
		+	=== Gel verification of Dave Lane plasmids (suite)===
		+	In a 1% agar gel, we have test pDAG464 and pDAG479 plasmids digested by XbaI and PstI enzyme. We've look if lines that we should see are the good one.
		+
		+	[[File:gelverif2dlplasmid.jpg|450px|thumb|center|Gel of verification Dave Lane plasmids digestion]]
		+
		+	*Column 1 : expected 450/833/950/1545/2110 pb
		+	*Column 2 : expected 402/833/950/1545/2110 pb
		+
		+	I don't understand we don't have what we expected. I will talk about it tomorrow with the team.

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Revision as of 16:51, 7 August 2011

Contents 1 Cyrille 2 Kevin 2.1 Miniprep from glycerol 2.2 Gel verification of Dave Lane plasmids (suite)

Cyrille

Treatement using inkscape of the gels done yesterday. In this picture, the progressive explanation of the identification of the bands.

So the miniprep 4 seems to contain the plasmid!

Kevin

Miniprep from glycerol

To re-test pDAG464 and pDAG479 plasmids, culture of them overnight has been done and miniprep. Quantification by TECAN :

• pDAG464 : 122,9 ng/uL
• pDAG479 : 204,9ng/uL

Gel verification of Dave Lane plasmids (suite)

In a 1% agar gel, we have test pDAG464 and pDAG479 plasmids digested by XbaI and PstI enzyme. We've look if lines that we should see are the good one.

• Column 1 : expected 450/833/950/1545/2110 pb
• Column 2 : expected 402/833/950/1545/2110 pb

I don't understand we don't have what we expected. I will talk about it tomorrow with the team.