Team:Paris Liliane Bettencourt/Notebook/2011/08/07/

From 2011.igem.org

Team IGEM Paris 2011

Contents

Cyrille

Treatement using inkscape of the gels done yesterday. In this picture, the progressive explanation of the identification of the bands.

Analysis

So the miniprep 4 seems to contain the plasmid with the EcoRI at the good place! To understand the process, here is the plasmid map.

pHM3 map

Kevin

Miniprep from glycerol

To re-test pDAG464 and pDAG479 plasmids, culture of them overnight has been done and miniprep. Quantification by TECAN :

  • pDAG464 : 122,9 ng/uL
  • pDAG479 : 204,9ng/uL

Gel verification of Dave Lane plasmids (suite)

In a 1% agar gel, we have test pDAG464 and pDAG479 plasmids digested by XbaI and PstI enzyme. We've look if lines that we should see are the good one.

Gel of verification Dave Lane plasmids digestion
  • Column 1 : expected 450/833/950/1545/2110 pb
  • Column 2 : expected 402/833/950/1545/2110 pb

I don't understand we don't have what we expected. I will talk about it tomorrow with the team.

Danyel & Camille

T7 amber

The digested PCR products were transformed in MH1 cells and plated, then incubated at 37ºC overnight.

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