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Team:Paris Bettencourt/Modeling/Parameters
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Revision as of 09:55, 12 September 2011 by BaptisteCouly (Talk | contribs)
Parameters
| Parameter | Description | Value | Unit | Reference |
|---|---|---|---|---|
 |
Concentration of X | NA | molecules per cell |
Notation convention |
 |
Delay due to protein X production and maturation | NA | s | Notation convention |
 |
Maximal production rate of pVeg promoter (constitutive) | ??? | molecules.s-1 or pops |
Estimated |
 |
Maximal production rate of pLac promoter | 0.02 | molecules.s-1 or pops |
Estimated |
 |
Maximal production rate of pT7 promoter | 0.02 | molecules.s-1 or pops |
Estimated |
 |
Dissociation constant for IPTG to LacI | 1200 | molecules per cell |
Aberdeen 2009 wiki |
 |
Dissociation constant for LacI to LacO (pLac) | 700 | molecules per cell |
Aberdeen 2009 wiki |
 |
Dissociation constant for T7 RNA polymerase to pT7 | 4.8 | molecules per cell |
Estimated ADD EXPLANATION |
 |
Translation rate of proteins | 0.9 | s-1 | Estimated, see the justification |
 |
Dilution rate in exponential phase | 2.88x10-4 | s-1 | Calculated with a 40 min generation time. See explanation |
 |
Degradation rate of mRNA | 2.88x10-3 | s-1 | Uri Alon (To Be Confirmed) |
 |
Delay due tT7 RNA polymerase production and maturation | 300 | s | http://mol-biol4masters.masters.grkraj.org/html/Prokaryotic_DNA_Replication13-T7_Phage_DNA_Replication.htm |
 |
Delay due to mRNA production | 30 | s | http://bionumbers.hms.harvard.edu/bionumber.aspx?s=y&id=104902&ver=5&hlid=58815 2kb/(50b/s) --> approximation: all our contructs are around 2kb |
