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Team:Paris Bettencourt/Experiments/YFP TetR diffusion experiments
From 2011.igem.org
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<p>We success to integrate the TetO Array in <i>B. subtilis</i> with the multihost episomal pHM3 plasmid. <br> | <p>We success to integrate the TetO Array in <i>B. subtilis</i> with the multihost episomal pHM3 plasmid. <br> | ||
| - | Experiments of E. coli (pFX234 plasmid, D. Lane strains) to B. subtilis (TetO Array, pHM3) to follow an eventual fluorescence diffusion by nanotube has been tried.</p> | + | Experiments of <i>E. coli</i> (pFX234 plasmid, D. Lane strains) to <i>B. subtilis</i> (TetO Array, pHM3) to follow an eventual fluorescence diffusion by nanotube has been tried.</p> |
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|+At the beginning of the movie | |+At the beginning of the movie | ||
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| - | |[[File:TetRTetO0_YFPTrans01.jpg|350px|thumb|center]] | + | |[[File:TetRTetO0_YFPTrans01.jpg|350px|thumb|center|Mix of <i>E. coli</i> (YFP:tetR) and <i>B. subtilis</i> (TetO Array) - Trans image]] |
| - | |[[File:TetRTetO0_YFPFluo01.jpg.jpg|350px|thumb|center]] | + | |[[File:TetRTetO0_YFPFluo01.jpg.jpg|350px|thumb|center|Mix of <i>E. coli</i> (YFP:tetR) and <i>B. subtilis</i> (TetO Array) - Fluo image]] |
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{| border="1" class="wikitable" style="text-align: center;" | {| border="1" class="wikitable" style="text-align: center;" | ||
|+At the end of the movie | |+At the end of the movie | ||
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| - | |[[File:TetRTetO0_YFPTrans02.jpg|350px|thumb|center]] | + | |[[File:TetRTetO0_YFPTrans02.jpg|350px|thumb|center|Mix of <i>E. coli</i> (YFP:tetR) and <i>B. subtilis</i> (TetO Array) - Trans image]] |
| - | |[[File:TetRTetO0_YFPFluo02.jpg|350px|thumb|center]] | + | |[[File:TetRTetO0_YFPFluo02.jpg|350px|thumb|center|Mix of <i>E. coli</i> (YFP:tetR) and <i>B. subtilis</i> (TetO Array) - Fluo image]] |
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Revision as of 22:51, 28 October 2011
Testing nanotubes with the YFP concentrator system
Summary
Results for the YFP concentrator:
- We successfully BioBricked both the YFP:TetR (BBa_K606025) and the TetO Array (BBa_K606026) constructs and sent them to the registry
- We characterized the YFP:TetR fusion protein both in E.coli and B.subtilis
- We characterized the TetO array both in E.coli and B.subtilis
- We've done E.coli to B.subtilis diffusion experiments (with negative results)
Design overview
YFP:TetR/TetO array system
More information on the design here.
Emitter & receiver constructs in B.subtilis (receiver in plasmid)
Emitter construct in E.coli - Receiver construct in B.subtilis (plasmid)
We success to integrate the TetO Array in B. subtilis with the multihost episomal pHM3 plasmid.
Experiments of E. coli (pFX234 plasmid, D. Lane strains) to B. subtilis (TetO Array, pHM3) to follow an eventual fluorescence diffusion by nanotube has been tried.
 |  |
 |  |
Movie : Experiment of mixed YFP:tetR (E. coli) & TetO Array (B. subtilis)
We cannot see spot apparitions specific of YFP concentration on TetO Array. This experiment leads us to think that there is no diffusion of the YFP:tetR fusion protein from E. coli to B. subtilis by nanotube.
