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Team:Paris Bettencourt/Experiments/List
From 2011.igem.org
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| - | <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/ | + | <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion"><img style="width:150px" src="https://static.igem.org/mediawiki/2011/d/d0/YFP_concentration_button.png"></a> |
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| - | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/ | + | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion">YFP concentration</a></b> This design relies on a TetO-array which allow us to concentrate YFP-TetR fusion proteins. |
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| - | <td style="width:200px; text-align:center;"><a href="https://2011.igem.org/Team:Paris_Bettencourt/T7_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/e/e4/T7_button.png"></a> | + | <td style="width:200px; text-align:center;"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/e/e4/T7_button.png"></a> |
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| - | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/T7_diffusion">T7 RNA polymerase diffusion</a></b> In this design, we introduce the use of the T7 polymerase both as the transfer molecule and as the auto-amplification system. | + | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/T7_diffusion">T7 RNA polymerase diffusion</a></b> In this design, we introduce the use of the T7 polymerase both as the transfer molecule and as the auto-amplification system. |
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| - | <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/tRNA_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/5/53/TRNAamber-button.png"></a> | + | <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/tRNA_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/5/53/TRNAamber-button.png"></a> |
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| - | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/tRNA_diffusion">tRNA amber diffusion</a></b> The tRNA amber is the smallest molecule we are trying to get pass the nanotubes. | + | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/tRNA_diffusion">tRNA amber diffusion</a></b> The tRNA amber is the smallest molecule we are trying to get pass the nanotubes. |
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| - | <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/ComS_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a> | + | <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/ComS_diffusion"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a> |
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| - | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/ComS_diffusion">ComS diffusion</a></b> We took advantage of a switch already existing in <i>B.Subtilis</i> (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes. | + | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/ComS_diffusion">ComS diffusion</a></b> We took advantage of a switch already existing in <i>B.Subtilis</i> (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes. |
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| - | <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/SinOp"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a> | + | <td style="width:200px; text-align:center"><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/SinOp"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a> |
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| - | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/SinOp">Sin Operon</a></b> | + | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/SinOp">Sin Operon</a></b> |
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| - | <td style="width:200px; text-align:center">Lambda switch<a href="https://2011.igem.org/Team:Paris_Bettencourt/Lambda_switch"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a> | + | <td style="width:200px; text-align:center">Lambda switch<a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/Lambda_switch"><img style="width:150px; margin-top:20px;" src="https://static.igem.org/mediawiki/2011/2/21/ComS-button.png"></a> |
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| - | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Lambda_switch">Lambda switch</a></b> | + | <td><b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/Lambda_switch">Lambda switch</a></b> |
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Revision as of 19:07, 20 September 2011
Experiments list
New devices
We designed entirely these new devices. They are usually composed of an emitter, a receptor and an amplifier sub-unit.
|
YFP concentration This design relies on a TetO-array which allow us to concentrate YFP-TetR fusion proteins. |
|
T7 RNA polymerase diffusion In this design, we introduce the use of the T7 polymerase both as the transfer molecule and as the auto-amplification system. |
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tRNA amber diffusion The tRNA amber is the smallest molecule we are trying to get pass the nanotubes. |
Using bistable switches
During our brainstormings, we noticed several natural or artificial bistable switches that could serve both as a receptor and an auto-amplifier. One molecule carefully chosen could toggle the switch in another position. All we have to do is see if it diffuses through the nanotubes.
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ComS diffusion We took advantage of a switch already existing in B.Subtilis (the ComK/ComS switch) and tried to see if we could toggle it from one state to the other using molecules diffusing through the nanotubes. |
|
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Sin Operon |
| Lambda switch
|
Lambda switch |
