Team:Paris Bettencourt/Experiments/Lambda switch

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Team IGEM Paris 2011

Building the Lambda switch design

Abstract

Results for the YFP concentrator:

  • We successfully BioBricked both the YFP:TetR (BBa_K606025) and the TetO Array (BBa_K606026) constructs and sent them to the registry
  • We integrated our construct both in E.coli and B.subtilis
  • We've done E.coli to B.subtilis diffusion experiments (with negative results)


Design overview

The lambda switch system principle

Integration into B. subtilis

Integration of cI (<partinfo>BBa_K606068</partinfo>)

We success to integrate BBa_K606068 into B. subtilis with an episomal vector K090401.

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