Team:Paris Bettencourt/Data

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Team IGEM Paris 2011

Data page

The YFP concentration system

The YFP concentration system principle

Data for our favorite new parts

  1. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.

Data for Pre-existing Parts

  1. Experience - Gram positive chromosomal integration plasmid, BBa_K090403 (Cambridge, iGEM 2008):



The T7 polymerase diffusion system

The T7 polymerase diffusion system principle

Data for our favorite new parts

  1. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. Main Page - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. Experience - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The tRNA Amber diffusion system

The tRNA Amber diffusion system principle

Data for our favorite new parts

  1. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. Main Page - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. Experience - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The ComS diffusion system

The ComS diffusion system principle

Data for our favorite new parts

  1. Main Page - ComS gene, BBa_K606037: The comS gene can trigger the MecA/ComS/ComK system. The start and stop codon has been changed for more standard codons.
  2. Main Page - ComS gene Codon optimized, BBa_K606038: This is the same gene than the previous one, but it has been synthetized de novo.

Data for Pre-existing Parts

  1. Main Page - pVeg + SpoVG, BBa_K143053 promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
  2. Main Page - Double standard terminator, BBa_B0015 standard double terminator
  3. Main Page - RFP, BBa_K143021 RFP m-cherry.
  4. Elowitz's pComG-CFP/pComS-YFP reporter strain - Competence reporter strain. Published in this paper [1]
  5. CodY- strain - From Abraham L. Sonenshein's laboratory, Tufts university [2]

The SinOp system

Data for our favorite new parts

  1. Main Page - KinA gene, BBa_K606046 KinA gene. SinOp kinase.
  2. Main Page - pVeg-SpoVg-KinA gene, BBa_K606047 KinA gene with B. subtilis promoter

Data for Pre-existing Parts

  1. SinOp reporter strain, given by J.W. Veening, published in this paper [3]

The lambda switch system

The lambda switch system principle

Data for our favorite new parts

  1. Main Page - pVeg SpoVg CI TT, BBa_K606044: Expression casette for for CI in B. subtilis.

Data for Pre-existing Parts

  1. Experience - pVeg + SpoVG, BBa_K143053 - (Imperial College, iGEM 2008)
  2. Main Page - Double standard terminator, BBa_B0015 - Standard double terminator.
  3. Main Page - CI, BBa_B0052 - CI gene from the lambda phage.



GFP diffusion

Nanotube and GFP.jpeg

Data for Pre-existing Parts

  1. Experience - Pveg-spoVG GFP expression construct, BBa_K143079 (Imperial College, iGEM 2008):



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