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Team:Paris Bettencourt/Data
From 2011.igem.org
Data page
The YFP concentration system
Data for our favorite new parts
- Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
Data for Pre-existing Parts
- Experience - Gram positive chromosomal integration plasmid, BBa_K090403 (Cambridge, iGEM 2008):
The T7 polymerase diffusion system
Data for our favorite new parts
- Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
- Main Page - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
- Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
Data for Pre-existing Parts
- Experience - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):
The tRNA Amber diffusion system
Data for our favorite new parts
- Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
- Main Page - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
- Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
Data for Pre-existing Parts
- Experience - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):
The ComS diffusion system
Data for our favorite new parts
- Main Page - ComS gene, BBa_K606037: The comS gene can trigger the MecA/ComS/ComK system. The start and stop codon has been changed for more standard codons.
- Main Page - ComS gene Codon optimized, BBa_K606038: This is the same gene than the previous one, but it has been synthetized de novo.
Data for Pre-existing Parts
- Main Page - pVeg + SpoVG, BBa_K143053 promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
- Main Page - Double standard terminator, BBa_B0015 standard double terminator
- Main Page - RFP, BBa_K143021 RFP m-cherry.
- Elowitz's pComG-CFP/pComS-YFP reporter strain - Competence reporter strain. Published in this paper [1]
- CodY- strain - From Abraham L. Sonenshein's laboratory, Tufts university [2]
The SinOp system
Data for our favorite new parts
- Main Page - KinA gene, BBa_K606046 KinA gene. SinOp kinase.
- Main Page - pVeg-SpoVg-KinA gene, BBa_K606047 KinA gene with B. subtilis promoter
Data for Pre-existing Parts
- SinOp reporter strain, given by J.W. Veening, published in this paper [3]
The lambda switch system
Data for our favorite new parts
- Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
- Main Page - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
- Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
GFP diffusion
Data for Pre-existing Parts
- Experience - Pveg-spoVG GFP expression construct, BBa_K143079 (Imperial College, iGEM 2008):
