Team:Paris Bettencourt/Data

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Team IGEM Paris 2011

Data page

The YFP concentration system

The YFP concentration system principle

Data for our favorite new parts

  1. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.

Data for Pre-existing Parts

  1. Experience - Gram positive chromosomal integration plasmid, BBa_K090403 (Cambridge, iGEM 2008):



The T7 polymerase diffusion system

The T7 polymerase diffusion system principle

Data for our favorite new parts

  1. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. Main Page - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. Experience - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The tRNA Amber diffusion system

The tRNA Amber diffusion system principle

Data for our favorite new parts

  1. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. Main Page - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. Experience - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The ComS diffusion system

The ComS diffusion system principle

Data for our favorite new parts

  1. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. Main Page - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. Experience - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):

The KinA system

To be completed

The lambda switch system

The lambda switch system principle

Data for our favorite new parts

  1. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. Main Page - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

GFP diffusion

Nanotube and GFP.jpeg

Data for Pre-existing Parts

  1. Experience - Pveg-spoVG GFP expression construct, BBa_K143079 (Imperial College, iGEM 2008):



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