Team:Paris Bettencourt/Data

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Team IGEM Paris 2011

Data page

The YFP concentration system : YFP:tetR and TetO array

The YFP concentration system principle

Data for our favorite new parts

  1. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.

Data for Pre-existing Parts

  1. Experience - Gram positive chromosomal integration plasmid, BBa_K090403 (Cambridge, iGEM 2008):



The T7 polymerase diffusion system

The T7 polymerase diffusion system principle

Data for our favorite new parts

  1. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. Main Page - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. Experience - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The tRNA Amber diffusion system

The tRNA Amber diffusion system principle

Data for our favorite new parts

  1. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. Main Page - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. Experience - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The ComS diffusion system

The ComS diffusion system principle

Data for our favorite new parts

  1. Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. Main Page - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. Main Page - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. Experience - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):

The KinA system

To complete

The lambda switch system

To complete

GFP diffusion

To complete



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