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Team:Paris Bettencourt/Data
From 2011.igem.org
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<h3>Data for our favorite new parts</h3> | <h3>Data for our favorite new parts</h3> | ||
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| - | # [http://partsregistry.org/Part: | + | # [http://partsregistry.org/Part:BBa_K606039:Experience Experience] – ''' |
| - | # [http://partsregistry.org/Part: | + | pVeg SpoVG T7 SpoVG RFP double terminator, BBa_K606039''' : T7 and RFP under constitutive promoter pVeg + SpoVG, emitter system to “send” the T7 polymerase. |
| - | + | # [http://partsregistry.org/Part:BBa_K606036:Experience Experience] – '''pT7 SpoVG T7 SpoVG GFP T7terminator''' : T7 and GFP under a pT7 promoter to response a emission of T7. | |
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<h2>The tRNA Amber diffusion system</h2> | <h2>The tRNA Amber diffusion system</h2> | ||
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| - | [[File:tRNA_amber_principle.jpg|center|The tRNA Amber diffusion system principle]] | + | [[File:tRNA_amber_principle.jpg|945px|center|The tRNA Amber diffusion system principle]] |
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<h3>Data for our favorite new parts</h3> | <h3>Data for our favorite new parts</h3> | ||
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| - | # [http://partsregistry.org/Part: | + | # [http://partsregistry.org/Part:BBa_K606040 Main Page] - '''Promoter Hyperspank, BBa_K606040''': IPTG Inducible promoter shortened compared to the one that was before in the registry. |
| - | # [http://partsregistry.org/Part: | + | # [http://partsregistry.org/Part:BBa_K606041 Main Page] - '''pHyperspank Tyrosine amber supressor tRNA double terminator, BBa_K606041''': system for using the tRNA in a IPTG inducible system. |
| - | # [http://partsregistry.org/Part: | + | # [http://partsregistry.org/Part:BBa_K606034 Main Page] - '''Tyrosine amber supressor tRNA for subtilis, BBa_K606034''': tRNA tyrosine amber compatible with both B. subtilis and E. coli. |
| + | # [http://partsregistry.org/Part:BBa_K606032 Main Page] - '''T7 amber, BBa_K606032''': This T7 polymerase contains two amber mutation to be a good receiver in a emitter/receiver system with a Tyrosine tRNA amber. | ||
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Revision as of 03:05, 22 September 2011
Data page
The YFP concentration system
Data for our favorite new parts
- Main Page - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
Data for Pre-existing Parts
- Experience - Gram positive chromosomal integration plasmid, BBa_K090403 (Cambridge, iGEM 2008):
The T7 polymerase diffusion system
Data for our favorite new parts
pVeg SpoVG T7 SpoVG RFP double terminator, BBa_K606039 : T7 and RFP under constitutive promoter pVeg + SpoVG, emitter system to “send” the T7 polymerase.
- Experience – pT7 SpoVG T7 SpoVG GFP T7terminator : T7 and GFP under a pT7 promoter to response a emission of T7.
The tRNA Amber diffusion system
Data for our favorite new parts
- Main Page - Promoter Hyperspank, BBa_K606040: IPTG Inducible promoter shortened compared to the one that was before in the registry.
- Main Page - pHyperspank Tyrosine amber supressor tRNA double terminator, BBa_K606041: system for using the tRNA in a IPTG inducible system.
- Main Page - Tyrosine amber supressor tRNA for subtilis, BBa_K606034: tRNA tyrosine amber compatible with both B. subtilis and E. coli.
- Main Page - T7 amber, BBa_K606032: This T7 polymerase contains two amber mutation to be a good receiver in a emitter/receiver system with a Tyrosine tRNA amber.
Data for Pre-existing Parts
- Experience - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):
The ComS diffusion system
Data for our favorite new parts
- Main Page - ComS gene, BBa_K606037: The comS gene can trigger the MecA/ComS/ComK system. The start and stop codon has been changed for more standard codons.
- Main Page - ComS gene Codon optimized, BBa_K606038: This is the same gene than the previous one, but it has been synthetized de novo.
Data for Pre-existing Parts
- Main Page - pVeg + SpoVG, BBa_K143053 promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
- Main Page - Double standard terminator, BBa_B0015 standard double terminator
- Main Page - RFP, BBa_K143021 RFP m-cherry.
- Elowitz's pComG-CFP/pComS-YFP reporter strain - Competence reporter strain. Published in this paper [1]
- CodY- strain - From Abraham L. Sonenshein's laboratory, Tufts university [2]
The SinOp system
Data for our favorite new parts
- Main Page - KinA gene, BBa_K606046 KinA gene. SinOp kinase.
- Main Page - pVeg-SpoVg-KinA gene, BBa_K606047 KinA gene with B. subtilis promoter
Data for Pre-existing Parts
- SinOp reporter strain, given by J.W. Veening, published in this paper [3]
The lambda switch system
Data for our favorite new parts
- Main Page - pVeg SpoVg CI TT, BBa_K606044: Expression casette for for CI in B. subtilis.
Data for Pre-existing Parts
- Experience - pVeg + SpoVG, BBa_K143053 - (Imperial College, iGEM 2008)
- Main Page - Double standard terminator, BBa_B0015 - Standard double terminator.
- Main Page - CI, BBa_B0052 - CI gene from the lambda phage.
GFP diffusion
Data for Pre-existing Parts
- Experience - Pveg-spoVG GFP expression construct, BBa_K143079 (Imperial College, iGEM 2008):
