Team:Paris Bettencourt/Data

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<h1>Data page</h1>
<h1>Data page</h1>
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<p><em>YFP:tetR</em> is a recombinant fusion protein. It is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO). Using the tetO array composed by a 10kb tetO sequence, we can concentrate YFP:tetR in a few loci and increase the fluorescence sensibility.
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<h2>The YFP concentration system : YFP:tetR and TetO array</h2>
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Origins of YFP:tetR and tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004)</p>
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<p> In the Ben-Yehuda paper, GFP has been proved to pass though the nanotubes.  We start to build the same experiment but improved by the tetR:YFP - tetO Array system and we used this design as a proof of nanotube concept between <i>B.Subtilis - B.Subtilis</i> and <i>B.Subtilis - E. Coli</i>.</p>
 
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<h2>Making the YFP:tetR diffuse through the tube</h2>
 
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<p><em>In the emittor cell <i>(B. Subtilis)</i></em>, we have inserted a expressive system for the YFP:tetR. It contains the promoter pVeg, the RBS for B. Subtilis and the YFP:tetR protein. Production of YFP:tetR will diffuse throught the nanotube to the receiver cell.</p>
 
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<p><em>In the receiver cell <i>(B. Subtilis or E. Coli)</i></em>, there is the tetO array where diffused YFP:tetR will concentrate. The YFP is the monitor of the signal.</p>
 
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<p>The principle of the design is summed up in the image below</p>
 
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<center><img src="https://static.igem.org/mediawiki/2011/5/56/TetR-YFP4.jpg">
<center><img src="https://static.igem.org/mediawiki/2011/5/56/TetR-YFP4.jpg">
<p><u>Fig1:</u> Schematics of the YFP concentration design</center></p>  
<p><u>Fig1:</u> Schematics of the YFP concentration design</center></p>  

Revision as of 12:38, 15 September 2011

Team IGEM Paris 2011