Team:Northwestern/Notebook/Protocols/Sequencing

From 2011.igem.org

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Use a 96 conical well plate. Each well has:
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Use a 96 conical well plate. Fill each well with:

Latest revision as of 14:59, 25 August 2011

RETURN TO IGEM 2010


DNA Sequencing


Use a 96 conical well plate. Fill each well with:


Forward primer 4 pM
Reverse primer 4 pM
DNA 200 ng
Nuclease-free H2O bring up final volume to 5 uL


Fill out spreadsheet from the [http://web.cgm.northwestern.edu/cgm/Core-Facilities/Genomics-Core Genomics Core Facility]