Team:Northwestern/Notebook/Protocols/Sequencing

From 2011.igem.org

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DNA Sequencing


Use a 96 conical well plate. Fill each well with:


Forward primer 4 pM
Reverse primer 4 pM
DNA 200 ng
Nuclease-free H2O bring up final volume to 5 uL


Fill out spreadsheet from the Genomics Core Facility