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Team:Northwestern/Notebook/Protocols/Sequencing
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DNA Sequencing</DIV> | DNA Sequencing</DIV> | ||
---------------------- | ---------------------- | ||
| + | |||
Use a 96 conical well plate. Each well has: | Use a 96 conical well plate. Each well has: | ||
| - | 4 pM | + | |
| - | 4 pM | + | :::{| border = "3" |
| - | 200 ng | + | |- |
| - | + | |align = "center" |Forward primer | |
| + | |align = "center" |4 pM | ||
| + | |- | ||
| + | |align = "center" |Reverse primer | ||
| + | |align = "center" |4 pM | ||
| + | |- | ||
| + | |align = "center" |DNA | ||
| + | |align = "center" |200 ng | ||
| + | |- | ||
| + | |align = "center" |Nuclease-free H2O | ||
| + | |align = "center" |bring up final volume to 5 uL | ||
| + | |- | ||
| + | |} | ||
Fill out spreadsheet from the [http://web.cgm.northwestern.edu/cgm/Core-Facilities/Genomics-Core Genomics Core Facility] | Fill out spreadsheet from the [http://web.cgm.northwestern.edu/cgm/Core-Facilities/Genomics-Core Genomics Core Facility] | ||
Revision as of 22:28, 23 August 2011
PROJECT
RESULTS
CONSIDERATIONS
ABOUT US
NOTEBOOK
ATTRIBUTIONS
DNA Sequencing
Use a 96 conical well plate. Each well has:
Forward primer 4 pM Reverse primer 4 pM DNA 200 ng Nuclease-free H2O bring up final volume to 5 uL
Fill out spreadsheet from the [http://web.cgm.northwestern.edu/cgm/Core-Facilities/Genomics-Core Genomics Core Facility]
