Team:Nevada/Project/Results

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Revision as of 02:15, 29 September 2011

Results Summary

E. coli

Cyanobacteria

Each gene part of the two knockout/operon insertion constructs has been successfully isolated and identified by gel electrophoresis. Additionally, the invertase (inv) and glucose-facilitator transport genes (GLF) were confirmed by sequence. The success rate of assembling gene parts by Gibson assembly is very low for two parts alone, let alone four. For this reason we have begun experiments using PCR to build the internal constructs of each KO/operon prior to attempting Gibson and other variations of "chewback" DNA technology. Furthermore, exploration of which enzymes are particularly well-suited for our unique constructs should lead to increased success rates.

@@ Line 13: / Line 13: @@
 === '''E. coli''' ===
 === '''Cyanobacteria''' ===
+Each gene part of the two knockout/operon insertion constructs has been successfully isolated and identified by gel electrophoresis. Additionally, the invertase (inv) and glucose-facilitator transport genes (GLF) were confirmed by sequence. The success rate of assembling gene parts by Gibson assembly is very low for two parts alone, let alone four. For this reason we have begun experiments using PCR to build the internal constructs of each KO/operon prior to attempting Gibson and other variations of "chewback" DNA technology. Furthermore, exploration of which enzymes are particularly well-suited for our unique constructs should lead to increased success rates.
 === '''Co-cultivation''' ===