Team:Nevada/Project
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'''ThiE Gene:''' The slr1176 open reading frame was PCR amplified from total ''Synechocystis'' PCC6803 genomic DNA and subcloned into pSB1a3. Forward and reverse primers were designed with 5’ extensions containing iGEM prefix and suffix sequences.<BR> | '''ThiE Gene:''' The slr1176 open reading frame was PCR amplified from total ''Synechocystis'' PCC6803 genomic DNA and subcloned into pSB1a3. Forward and reverse primers were designed with 5’ extensions containing iGEM prefix and suffix sequences.<BR> | ||
- | '''Glucose facilitative transporter (GLF) Gene+double terminator:""" The GLF gene was synthesized based on the sequence of a ''Zymonomas mobilis'' invertase gene described by Neiderholtmeyer et al. (Applied and Environmental Microbiology (2010) 76: 3462) which was codon optimized for expression in cyanobacteria. The gene was also synthesized with a double transcriptional terminator designed from sequences described for iGEM part | + | '''Glucose facilitative transporter (GLF) Gene+double terminator:""" The GLF gene was synthesized based on the sequence of a ''Zymonomas mobilis'' invertase gene described by Neiderholtmeyer et al. (Applied and Environmental Microbiology (2010) 76: 3462) which was codon optimized for expression in cyanobacteria. The gene was also synthesized with a double transcriptional terminator designed from sequences described for iGEM part BBa_KK0005.<BR> |
'''petBD promoter+RBS:''' The strong light inducible petBD promoter will be used to drive the expression of invertase gene in Synechocystis. The petBD+RBS (BBa_K390015) was generously provided to us by the Utah State iGEM team.<BR> | '''petBD promoter+RBS:''' The strong light inducible petBD promoter will be used to drive the expression of invertase gene in Synechocystis. The petBD+RBS (BBa_K390015) was generously provided to us by the Utah State iGEM team.<BR> |
Revision as of 05:44, 26 September 2011
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