Team:NYMU-Taipei/results/chassis1

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AMB-1's growth curve When is the Log phase?

AMB-1 growth curve with MSGM.
The Horizontal axis means the time of growth and the Vertical axis means the OD565, the maximum absorbance of AMB-1. The AMB-1 enters log phase at about 7~10 days.

We present AMB-1's growth curve in terms of OD565, the maximum absorbance of AMB-1. It is important to find out the log phase, because log phase is the best time for electroporation. We better keep AMB-1 in "log phase", because this phase is the index to do electroporation. The success rate will be greatly improved in log phase. Therefore, by keeping AMB-1 in "log phase" (approximately 3~6 days in Enrich-MSGM, 7~10 days in MSGM), we can get the best results from electroporation. After seven days, the bacterium would be into stationary phase. During the phase, the growth rate slows as a result of nutrient depletion and accumulation of toxic products. This phase is reached as the bacteria begin to exhaust the resources that are available to them. This phase is a constant value as the rate of bacterial growth is equal to the rate of bacterial death. So, if we want to get better quality of electroporation. we'd better make sure there are enough AMB-1 strain in log phase.