Team:NYMU-Taipei/results/chassis1
From 2011.igem.org
AMB-1's growth curve When is the Log phase?
We present AMB-1's growth curve in terms of OD565, the maximum absorbance of AMB-1. It is important to find out the log phase, because log phase is the best time for electroporation. We better keep AMB-1 in "log phase", because this phase is the index to do electroporation. The success rate will be greatly improved in log phase. Therefore, by keeping AMB-1 in "log phase" (approximately 3~6 days), we can get the best results from electroporation. After seven days, the bacterium would be into stationary phase. During the phase, the growth rate slows as a result of nutrient depletion and accumulation of toxic products. This phase is reached as the bacteria begin to exhaust the resources that are available to them. This phase is a constant value as the rate of bacterial growth is equal to the rate of bacterial death.