Team:NTNU Trondheim/LacI+pLac+mCherry

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Stress sensor precursor (LacI+pLac+mCherry)

LacI+pLac+mCherry rosa.png

This is our system without the rrnB P1-promotor. Because this construct lacks a downregulated promotor in front of it, LacI will not be syntethized, and in response, pLac will not be downregulated of LacI. Since pLac is not downregulated, mCherry is going to be present whether the cells are stressed or not. Our experiments indicate that the stress sensor precursor is put together the right way. The stress sensor precursor is made from the following biobricks:

Five parallels digested with BsaAI + HpaI and BstBI SpeI
Biobrick Part number
LacI with RBS BBa_J24679
Terminator BBa_B0015
pLac BBa_R0011
mCherry with RBS and terminator BBa_J06702


The composite biobrick has a total length of 2153 bp, and is located in the plasmid pSB1A2. In order the check if the construct was correct we digested the plasmid with BsaAI + HpaI and BstBI SpeI

This construct could in theory be used for any kind of biosensor. The only thing needed is a promotor that gets downregulated directly or indirectly by the conditions one would want to detect.

Link to partsregistry: [http://partsregistry.org/Part:BBa_K639000 BBa_K639000]