Team:Minnesota/leex4462-04212011000000-33

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== Miniprep, Restriction Digest of OmpA and pucBB, and Gel extraction == Project: Surface Display Date: Thu, 21 Apr 2011 00:00:00 GMT Author: Sukdong Lee (leex4462) Access: Public Revision History:
  • Mon, 22 Aug 2011 10:15:27 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
Purpose: Extract the pBAD plasmid from JM109, digest the of insert and vector and purify the cut DNA. Procedures: -Same steps as before. -Another liquid culture of the pBAD was inoculated due to the low yields. Results: Tube 2 (insert)= 21.9 ng/ul Plasmid DNA(undigested miniprep)= 12.9
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