Team:Minnesota/dingx055-04262011160253-154

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== PCR  trial #2 ==

'''Project:''' Surface Display

'''Date:''' Tue, 26 Apr 2011 16:02:53 GMT

'''Author:''' Viki Ding (dingx055)

'''Access:''' Public

'''Revision History:'''

:* Mon, 26 Sep 2011 16:05:57 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)<!-- Generated by Rtf2WikiMarkup - RTF to Wiki Markup Converter 1.0, Copyright © Phyrstorm Technologies, Inc. 2011 -->
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<nowiki>50 uL reaction</nowiki>

<nowiki>same volumes as before (4/26)</nowiki>



<nowiki>PCR conditions</nowiki>



<nowiki>For GFP:</nowiki>

<nowiki>95degrees >> 4min</nowiki>

<nowiki>95degrees >> 30 sec</nowiki>

<nowiki>60degrees >> 30 sec</nowiki>

<nowiki>72 degrees >> 45 sec</nowiki>

<nowiki>72 degrees >> 10min</nowiki>

<nowiki>34 cycles</nowiki>



<nowiki>For INP:</nowiki>

<nowiki>95degrees >> 4min</nowiki>

<nowiki>95degrees >> 30 sec</nowiki>

<nowiki>55degrees >> 30 sec</nowiki>

<nowiki>72 degrees >> 45 sec</nowiki>

<nowiki>72 degrees >> 10min</nowiki>

<nowiki>34 cycles</nowiki>



<nowiki>Results: multiple bands, not the correct size. </nowiki>


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