Team:Minnesota/dingx055-04262011155222-153

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PCR set up

Project: Surface Display

Date: Tue, 26 Apr 2011 15:52:22 GMT

Author: Viki Ding (dingx055)

Access: Public

Revision History:

  • Mon, 26 Sep 2011 16:00:18 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)
  • Mon, 26 Sep 2011 16:02:39 GMT (dingx055): entry data updated by dingx055 (id=9)

PCR set up for INPNC & GFP.


50 uL Reaction


1 µl vent polymerase

2 µl dNTP

2 µl DNA template

5 µl vent buffer

5 µl forward primer

5 µl reverse primer

30 µl nuclease free water

PCR conditions

95 degrees >> 10 min

95 degrees >> 30 sec

60 degrees >> 1 min

72 degrees >> 1 min

72 degrees >> 10 min

35 cycles

Results: multitple bands, not the correct size



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