Team:Minnesota/davis950-09092011114517-118

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== Miniprep of pUC-pcyA-ho1 == Project: Regulatory Date: Fri, 09 Sep 2011 11:45:17 GMT Author: Nathan Davis (davis950) Access: Public Revision History:
  • Fri, 09 Sep 2011 11:48:20 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)
Purpose: to isolate pUC-pcyA-ho1 plasmid from 4 3ml overnight cultures. Procedure: used Promega Wizard Plus miniprep kit according to manufacturer specifications. Results: Colony 1 has a concentration of 139.3 ng/ul Colony 2 has a concentration of 122.5 ng/ul Colony 3 has a concentration of 57.2 ng/ul Colony 4 has a concentration of 118.3 ng/ul Using Colony 1 from amp plate 1 and Colony 4 from plate 2 for digest.
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