Team:Minnesota/davis950-08302011132913-115

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<br>== PCR of ompR and silicatein ==
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== PCR of ompR and silicatein ==
'''Project:''' Regulatory
'''Project:''' Regulatory
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<nowiki>Silicatein alpha amplified from TOPO-Silicatein using the same reaction mixture as above.</nowiki>
<nowiki>Silicatein alpha amplified from TOPO-Silicatein using the same reaction mixture as above.</nowiki>
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{{Team:Minnesota/Bottom}}
{{Team:Minnesota/Bottom}}

Latest revision as of 22:46, 27 September 2011

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PCR of ompR and silicatein

Project: Regulatory

Date: Tue, 30 Aug 2011 13:29:13 GMT

Author: Nathan Davis (davis950)

Access: Public

Revision History:

  • Tue, 30 Aug 2011 13:33:57 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)

Set up a PCR reaction to amplify ompR promoter from distribution plates (BBa_R0082). Fifty ul reaction volume contained:


  • 1 ul template DNA
  • 1 ul forward primer
  • 1 ul reverse primer
  • 5 ul thermopol buffer
  • 0.5 ul 20 mM dNTPs
  • 2.5 ul 10 mM MgSO4
  • 0.5 ul Vent polymerase
  • 38.5 ul of H2O


Silicatein alpha amplified from TOPO-Silicatein using the same reaction mixture as above.


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