Team:Lyon-INSA-ENS/Realisation/Protocols/Ozyme

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Ozyme digestion





This protocol aims at cutting plasmids on specific restriction sites using restriction enzymes.





Procedure



1. In an eppendorf tube, add the following solutions in any order :
-11µL water
-10µL DNA
-2.5µL NE Buffer 2
-0.5µL BSA X50


2. Add 0.5µL of each one of the desired enzymes.


3. Incubate at 37°C for 10mn


4. Inactivate the enzymes by a 20mn incubation at 80°C








ENS assystem Biomérieux INSA INSA