"
Team:Kyoto
From 2011.igem.org
(Difference between revisions)
(→Human Practice) |
Grassfield (Talk | contribs) (→Achievement) |
||
| (16 intermediate revisions not shown) | |||
| Line 8: | Line 8: | ||
As such we named it Carnivorous E.coli. | As such we named it Carnivorous E.coli. | ||
Carnivorous E.coli emits light when it is hungry. This will attract insects to it. | Carnivorous E.coli emits light when it is hungry. This will attract insects to it. | ||
| - | Then, it secretes a viscous substance to | + | Then, it secretes a viscous substance to capture the insects. |
| - | The insects | + | The captured insects are digested by the chitinase in the secretion. |
Long story short, our Carnivorous E.coli can hunt! | Long story short, our Carnivorous E.coli can hunt! | ||
<!-- https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png --> | <!-- https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png --> | ||
| Line 18: | Line 18: | ||
== <html><a href="/Team:Kyoto/Hunger"><span class="red_title">Team Hunger</span></a></html> == | == <html><a href="/Team:Kyoto/Hunger"><span class="red_title">Team Hunger</span></a></html> == | ||
<html><div id="hunger"><a href="/Team:Kyoto/Hunger"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html> | <html><div id="hunger"><a href="/Team:Kyoto/Hunger"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html> | ||
| - | + | Carnivorous E.coli attracts insects by emitting light, but it is a burden for ''E.coli''. | |
| - | + | To reduce this burden, we use nitrogen regulatory proteins, NtrB and NtrC. They activate σ<sup>54</sup> promoter when the supply of nitrogen is not enough. | |
</div> | </div> | ||
<div> | <div> | ||
| - | == <html><a href="/Team:Kyoto/ | + | == <html><a href="/Team:Kyoto/Capture"><span class="green_title">Team Capture</span></a></html> == |
| - | <html><div id="capture"><a href="/Team:Kyoto/ | + | <html><div id="capture"><a href="/Team:Kyoto/Capture"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html> |
| + | There are many methods to attract bugs, for instance, using pheromone, smell, and light. | ||
| + | In this study, we focused on light as a method to lure insects. | ||
| + | Carnivorous E.coli will emit light and attracts bugs like glowworms by using Bioluciferase from <html><a href="https://2010.igem.org/Team:Cambridge">2010 Cambridge</a></html>. | ||
| + | <!-- | ||
There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone. | There are many ways to attract bugs, for instance using pheromone, but it is difficult for E.coli to synthesize complex compounds like pheromone. | ||
Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from | Carnivorous E.coli emits light and attracts bugs like glowworms by using Bioluciferase from | ||
<html><a href="https://2010.igem.org/Team:Cambridge">2010 Cambridge</a></html>. | <html><a href="https://2010.igem.org/Team:Cambridge">2010 Cambridge</a></html>. | ||
| + | --> | ||
</div> | </div> | ||
| Line 35: | Line 40: | ||
== <html><a href="/Team:Kyoto/Digestion"><span class="blue_title">Team Digestion</span></a></html> == | == <html><a href="/Team:Kyoto/Digestion"><span class="blue_title">Team Digestion</span></a></html> == | ||
<html><div id="digestion"><a href="/Team:Kyoto/Digestion"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html> | <html><div id="digestion"><a href="/Team:Kyoto/Digestion"><img src="https://static.igem.org/mediawiki/2011/3/3f/Kyoto_Project_Image.png"></img></a></div></html> | ||
| - | An insect body is covered with a hard integument containing mainly chitin. To decompose the integument, we used ChiA | + | An insect body is covered with a hard integument containing mainly chitin. To decompose the integument, we used ChiA gene, which encode chitinase. In order to measure the chitinase activity of the culture supernatant, we evaluated the effects of the medium and cell growth. |
</div> | </div> | ||
</div> | </div> | ||
| Line 69: | Line 74: | ||
<div id="under_box"> | <div id="under_box"> | ||
<div> | <div> | ||
| - | |||
| + | ==<span class="red_title">Achievement</span>== | ||
| + | <ul> | ||
| + | <li>We confirmed that the wavelength of light emitted by E.coli is suitable to lure drosophilas.</li> | ||
| + | <li>We constructed new biobrick which produces chitinase.</li> | ||
| + | <li>We constructed new biobrick that is controlled by the concentration of glutamine.</li> | ||
| + | <li>We established new method for measurement of promoter activity with RPU.</li> | ||
| + | <li>We held <html><a href="https://2011.igem.org/Summer_events/2011_JAPAN_Meetup">the Japanese iGEM meeting</a></html> at Kyoto University. | ||
| + | </ul> | ||
</div> | </div> | ||
<div> | <div> | ||
| + | |||
==<html><a href="https://2011.igem.org/Team:Kyoto/HumanPractice"><span class="green_title">Human Practice</span></a></html>== | ==<html><a href="https://2011.igem.org/Team:Kyoto/HumanPractice"><span class="green_title">Human Practice</span></a></html>== | ||
| - | As synthetic biology is a comparatively new way of understanding life, we plan to develop this area much further. However,this area entails problems of bioethics and so we must consider these problems as well. Unfortunately, Japanese high school students do not have many chances to come across these topics. We hope to offer high school students more opportunities to think on them. That's why we targeted them and performed these activities. | + | As synthetic biology is a comparatively new way of understanding life, we plan to develop this area much further. However, this area entails problems of bioethics and so we must consider these problems as well. Unfortunately, Japanese high school students do not have many chances to come across these topics. We hope to offer high school students more opportunities to think on them. That's why we targeted them and performed these activities. |
</div> | </div> | ||
<div> | <div> | ||
Latest revision as of 03:58, 6 October 2011
Contents |
Summary
We created new E.coli which hunts and eats insects. As such we named it Carnivorous E.coli. Carnivorous E.coli emits light when it is hungry. This will attract insects to it. Then, it secretes a viscous substance to capture the insects. The captured insects are digested by the chitinase in the secretion. Long story short, our Carnivorous E.coli can hunt!
Team Hunger
Team Capture
Team Digestion
Achievement
- We confirmed that the wavelength of light emitted by E.coli is suitable to lure drosophilas.
- We constructed new biobrick which produces chitinase.
- We constructed new biobrick that is controlled by the concentration of glutamine.
- We established new method for measurement of promoter activity with RPU.
- We held the Japanese iGEM meeting at Kyoto University.
Human Practice
As synthetic biology is a comparatively new way of understanding life, we plan to develop this area much further. However, this area entails problems of bioethics and so we must consider these problems as well. Unfortunately, Japanese high school students do not have many chances to come across these topics. We hope to offer high school students more opportunities to think on them. That's why we targeted them and performed these activities.
Sponsors
