Team:KIT-Kyoto/Notebook/LabNoteJ/DIAP2-MALTJ8
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8月8日
Member
- 松浪、横井川
- API2-MALT1、DIAP2について以下のプライマーを用いて、以下の条件でPCRを行った。
- API2-MALT1
- F primer:GCCGCTCGAGAACATAGTAGAAAACAGCAT
- Tm値 57.8°C
- R primer:GCCGGCTAGCTCATTTTTCAGAAATTCTGA
- Tm値 56.5°C
- 増幅サイズ 約3131 bp
- F primer:GCCGCTCGAGAACATAGTAGAAAACAGCAT
- DIAP2
- F primer:GCTTCTCGAGACGGAGCTGGGCATGGAGCT
- Tm値 69°C
- R primer:GCCGTCTAGATCACGAAAGGAACGTGCGCA
- Tm値 66.4°C
- 増幅サイズ 約1514 bp
- F primer:GCTTCTCGAGACGGAGCTGGGCATGGAGCT
- 1.API2-MALT1
PCR条件 10 µM Primer F 0.4 µl 10 µM Primer R 0.4 µl Template DNA 1 µl 10 x Ex Taq Buffer 2 µl Takara Ex Taq 0.1 µl 2.0 mM dNTPs 2 µl ddH2O 14.1 µl total 20 µl Cycle条件 Pre-Denature 94°C 2min Denature 94°C 30sec 37 Cycle Anneling 51.5°C 30sec Extension 72°C 3min20sec +Extension 72°C 10min End 4°C keep
- 2.DIAP2
PCR条件 10 µM Primer F 0.4 µl 10 µM Primer R 0.4 µl Template DNA 1 µl 10 x Ex Taq Buffer 2 µl Takara Ex Taq 0.1 µl 2.0 mM dNTPs 2 µl ddH2O 14.1 µl total 20 µl Cycle条件 Pre-Denature 94°C 2min Denature 94°C 30sec 37 Cycle Anneling 56.9°C 30sec Extension 72°C 1min40sec +Extension 72°C 10min End 4°C keep