Team:Johns Hopkins/YT/Future

From 2011.igem.org

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We plan to finish ligating the 5 violacein expression cassettes into the PRS416 Yeast Vector and transform it into a URA3 Knockout yeast strain.
We plan to finish ligating the 5 violacein expression cassettes into the PRS416 Yeast Vector and transform it into a URA3 Knockout yeast strain.
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We plan to carry out the random ligation to make the promoter/violacein ORF/3'UTR/loxPsym combinatorial library and transform into synIXR yeast to get varying levels of violacein expression.
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Revision as of 04:10, 27 September 2011

VitaYeast - Johns Hopkins University, iGEM 2011

Violacein

We plan to finish ligating the 5 violacein expression cassettes into the PRS416 Yeast Vector and transform it into a URA3 Knockout yeast strain.

We plan to carry out the random ligation to make the promoter/violacein ORF/3'UTR/loxPsym combinatorial library and transform into synIXR yeast to get varying levels of violacein expression.