Promoters and UTRs
The iGEM registry is lacking in promoter and terminators sequences for yeast, especially when compared with the number available for use in E. coli. In order to address this problem and increase the use of yeast in synthetic biology we have decided to provide a library of promoter and terminator sequences from 12 yeast genes: 4 causing high expression, 4 causing medium expression, and 4 causing low expression. We hope these BioBrick compatible parts will make it easier for future iGEM teams to work with Saccharomyces cerevisiae.
Promoters
Yeast promoter sequences of varying strength have been provided for our work. The following are the available promoters:
RPS8Bp | BAP2p | FCY2p | Gal 1/10p | HHO1p |
---|---|---|---|---|
KRE9p | PRY1p | STM1p | TDH3p | RPS2p |
UTRs
Similarly, the following UTRs, or termination sequences have been provided for our work:
ARD1utr | BAP2utr | FCY2utr | HHo1utr | KRE9utr |
---|---|---|---|---|
PRY1utr | RPL8Autr | RPL24Autr | RPS2utr | RPS8Butr |
TDH3utr |