Team:Johns Hopkins/Project/PromUTR

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======Promoters and UTRs======
======Promoters and UTRs======
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As we began our work with ''Saccharomyces cerevisiae'', it came to our attention that the database is deficient in genes for this organism. This deficiency is due in part to the fact that ''E. coli'' genes are widespread through the database, whereas yeast remains a largely untapped area. In order to address this problem and facilitate the use of yeast in synthetic biology we have decided to provide the basic parts required for gene use and assembly. We constructed some basic parts including promoters and termination sequences of varying strengths. This will allow future teams the ease of use of premade biobricked sequences to use with their genes.
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The iGEM registry is lacking in promoter and terminators sequences for yeast, especially when compared with the number available for use in ''E. coli''. In order to address this problem and increase the use of yeast in synthetic biology we have decided to provide a library of promoter and terminator sequences from 12 yeast genes: 4 causing high expression, 4 causing medium expression, and 4 causing low expression. We hope these BioBrick compatible parts will make it easier for future iGEM teams to work with ''Saccharomyces cerevisiae''.
======Promoters======
======Promoters======

Revision as of 05:23, 28 September 2011

VitaYeast - Johns Hopkins University, iGEM 2011

Promoters and UTRs

The iGEM registry is lacking in promoter and terminators sequences for yeast, especially when compared with the number available for use in E. coli. In order to address this problem and increase the use of yeast in synthetic biology we have decided to provide a library of promoter and terminator sequences from 12 yeast genes: 4 causing high expression, 4 causing medium expression, and 4 causing low expression. We hope these BioBrick compatible parts will make it easier for future iGEM teams to work with Saccharomyces cerevisiae.

Promoters

Yeast promoter sequences of varying strength have been provided for our work. The following are the available promoters:

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530016 RPS8Bp] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530005 BAP2p] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530015 FCY2p] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530018 Gal 1/10p] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530010 HHO1p]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530003 KRE9p] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530007 PRY1p] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530004 STM1p] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530008 TDH3p] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530009 RPS2p]
UTRs

Similarly, the following UTRs, or termination sequences have been provided for our work:

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530020 ARD1utr] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530006 BAP2utr] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530017 FCY2utr] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530019 HHo1utr] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530014 KRE9utr]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530013 PRY1utr] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530011 RPL8Autr] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530021 RPL24Autr] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530024 RPS2utr] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K530022 RPS8Butr]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K530023 TDH3utr]


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