Team:Imperial College London/Project Chemotaxis Future

From 2011.igem.org

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<h1>Future Work</h1>
<h1>Future Work</h1>
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<p>1. Repeat the uptake experiment in non-sterile conditions in soil in order to assess if uptake of bacteria is likely to happen in nature. This is especially important as the fine tuning of auxin expression depends on how the compound is administered to the plant.
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<p>To carry on the work on the Phyto-Xpress module, there are a number of steps we would take in the immediate future and others that form part of our long term plan.
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<h2>Short-term plans</h2>
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<p><b>1.Repeat the uptake experiment in non-sterile conditions in soil in order to assess if uptake of bacteria is likely to happen in nature. This is especially important as the fine tuning of auxin expression depends on how the compound is administered to the plant. </b>
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<p><b>2. Replicates of the soil experiment will be analysed for expression of sfGFP.</p></b>
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<p><b>3. </b>
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<h2>Long-term plans</h2>
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<p><b>1.  </b>
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<p><b>2. </b>
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<p><b>3.Testing and implementation in the field. </b></p>
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Revision as of 20:08, 21 September 2011




Module 1: Phyto-Route

Chemotaxis is the movement of bacteria based on attraction or repulsion of chemicals. Roots secrete a variety of compounds that E. coli are not attracted to naturally. Accordingly, we engineered a chemoreceptor into our chassis that can sense malate, a common root exudate, so that it can swim towards the root. Additionally, E. coli are actively taken up by plant roots, which will allow targeted IAA delivery into roots by our system.






Future Work

To carry on the work on the Phyto-Xpress module, there are a number of steps we would take in the immediate future and others that form part of our long term plan.

Short-term plans

1.Repeat the uptake experiment in non-sterile conditions in soil in order to assess if uptake of bacteria is likely to happen in nature. This is especially important as the fine tuning of auxin expression depends on how the compound is administered to the plant.

2. Replicates of the soil experiment will be analysed for expression of sfGFP.

3.

Long-term plans

1.

2.

3.Testing and implementation in the field.