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From 2011.igem.org

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Module 2: Auxin Xpress

Auxin, or Indole 3-acetic acid (IAA), is a plant growth hormone which is produced by several soil bacteria. We have taken the genes encoding the IAA-producing pathway from Pseudomonas savastanoi and expressed them in Escherichia coli. Following chemotaxis towards the roots and uptake by the Phyto Route module, IAA expression will promote root growth with the aim of improving soil stability.

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Specifications

1.A simple auxin producing pathway endogenous to bacteria to minimize assembly steps.

2.Minimizing synthesis costs while designing fragment sequences amenable to Gibson and CPEC assembly.

3.Achieving adequate auxin expression levels in our chassis to enhance root growth in our plant model.

4.Modularity of parts to facilitate use in different devices and chassis.