Team:Imperial College London/Project Auxin Specifications

From 2011.igem.org

(Difference between revisions)
Line 5: Line 5:
<body>
<body>
<h1>Specifications</h1>
<h1>Specifications</h1>
-
<p>The IAM pathway taken for this module is from Pseudomonas savastanoi. This strain of soil dwelling bacteria is a known plant pathogen that uses IAA to infect its target. However, there have been some recent studies that suggest that IAA secretion by bacteria can also lead to positive microbe-plant relations [1]. Therefore, we must carefully analyze what IAA concentration would aid root growth rather than promote gall formation. To achieve this, we will be experimenting with different levels of synthetic auxin on Arabidopsis thaliana. We will also be modelling this module in order to obtain the adequate concentration of IAA excretion from the chassis.</p>
+
<p>1.A simple auxin producing pathway endogenous to bacteria to minimize assembly steps.</p>
-
<p>Indole-3 acetic acid (IAA) is one of the most well studied phytohormones and is also known more commonly under the name Auxin. IAA is known as a key player in the regulation of plant growth and is also a known morphogen implicated in a vast array of processes ranging from embryo patterning to isodiametric expansion (fruit growth).</p>
+
<p>2.Minimizing synthesis costs while designing fragment sequences amenable to Gibson and CPEC assembly.</p>  
-
<p>However, the topic of auxin producing soil bacteria in the rhizosphere has been given little attention so far. We believe that plant-microbe interactions mediated through IAA could be tapped into to modulate the plasticity of the root architecture. In this module, we will be attempting to express Tryptophan monooxygenase (IaaM) and Idoleacetimide hydrolase (IaaH) in Escherichia coli. We are aware that E. coli would not be a suitable chassis for field work and we have taken this into account when we made our DNA sequences.</p>
+
<p>3.Achieving adequate auxin expression levels in our chassis to enhance root growth in our plant model. </p>
 +
<p>4.Modularity of parts to facilitate use in different devices and chassis. </p>
</body>
</body>
</html>
</html>

Revision as of 22:32, 15 September 2011



Module 2: Auxin Xpress

Auxin, or Indole 3-acetic acid (IAA), is a plant growth hormone which is produced by several soil bacteria. We have taken the genes encoding the IAA-producing pathway from Pseudomonas savastanoi and expressed them in Escherichia coli. Following chemotaxis towards the roots and uptake by the Phyto Route module, IAA expression will promote root growth with the aim of improving soil stability.



Specifications

1.A simple auxin producing pathway endogenous to bacteria to minimize assembly steps.

2.Minimizing synthesis costs while designing fragment sequences amenable to Gibson and CPEC assembly.

3.Achieving adequate auxin expression levels in our chassis to enhance root growth in our plant model.

4.Modularity of parts to facilitate use in different devices and chassis.

Retrieved from "http://2011.igem.org/Team:Imperial_College_London/Project_Auxin_Specifications"