Team:Harvard/Technology

From 2011.igem.org

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Check out our Zinc Finger Binding Site Finder Tool [[Team:Harvard/ZF_Binding_Site_Finder|here]]!  This tool was used to search the human genome for the six target DNA sequences that we used to design our custom zinc finger arrays.
Check out our Zinc Finger Binding Site Finder Tool [[Team:Harvard/ZF_Binding_Site_Finder|here]]!  This tool was used to search the human genome for the six target DNA sequences that we used to design our custom zinc finger arrays.
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=MAGE=
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*Source: http://www.nature.com/nature/journal/v460/n7257/full/nature08187.html
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[https://2011.igem.org/Team:Harvard/Results/Lambda_Red lambda red results]
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[https://2011.igem.org/Team:Harvard/Technology/Lambda_Red lambda red tech]
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[https://2011.igem.org/Team:Harvard/Technology/One-Hybrid_Selection_System One-Hybrid Selection System]
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[https://2011.igem.org/Team:Harvard/Technology/Zinc_Finger_Expression_Plasmids Zinc Finger Expression Plasmids]
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[https://2011.igem.org/Team:Harvard/Technology/MAGE MAGE tech]
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[https://2011.igem.org/Team:Harvard/Results/MAGE MAGE results]
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[https://2011.igem.org/Team:Harvard/ZF_Binding_Site_Finder ZFP binding site finder]
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[https://2011.igem.org/Team:Harvard/Protocols Protocols]
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=Lambda Red- Mediated Recombineering=
=Lambda Red- Mediated Recombineering=
==Useful Links==
==Useful Links==
*Gene Knockouts and Exchanges by Linear Transformation: http://rothlab.ucdavis.edu/protocols/Lin.Transform.html
*Gene Knockouts and Exchanges by Linear Transformation: http://rothlab.ucdavis.edu/protocols/Lin.Transform.html
*Open Wet Ware Protocol: http://openwetware.org/wiki/Recombineering/Lambda_red-mediated_gene_replacement
*Open Wet Ware Protocol: http://openwetware.org/wiki/Recombineering/Lambda_red-mediated_gene_replacement
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=Gibson (Isothermal) Assembly=
=Gibson (Isothermal) Assembly=
*Source: http://www.nature.com/nmeth/journal/v6/n5/full/nmeth.1318.html
*Source: http://www.nature.com/nmeth/journal/v6/n5/full/nmeth.1318.html
*Protocol: http://www.nature.com/protocolexchange/protocols/554#/main
*Protocol: http://www.nature.com/protocolexchange/protocols/554#/main
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Revision as of 22:07, 28 September 2011

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Overview | MAGE | Chip-Based Synthesis | Lambda Red | Protocols

Our project uses 3 major new technologies: Multiplex Automated Genome Engineering (MAGE), chip-based synthesis of DNA, and lamba red recombination, along with more traditional bioinformatics. We hope that future iGEM teams will also use these techniques in their own synthetic biology projects.

Bioinformatics

See our bioinformatics page for details on the computer aspects of our technology.

Zinc Finger Binding Site Finder

Check out our Zinc Finger Binding Site Finder Tool here! This tool was used to search the human genome for the six target DNA sequences that we used to design our custom zinc finger arrays.

MAGE

  • Source: http://www.nature.com/nature/journal/v460/n7257/full/nature08187.html

Lambda Red- Mediated Recombineering

Useful Links

  • Gene Knockouts and Exchanges by Linear Transformation: http://rothlab.ucdavis.edu/protocols/Lin.Transform.html
  • Open Wet Ware Protocol: http://openwetware.org/wiki/Recombineering/Lambda_red-mediated_gene_replacement

Gibson (Isothermal) Assembly

  • Source: http://www.nature.com/nmeth/journal/v6/n5/full/nmeth.1318.html
  • Protocol: http://www.nature.com/protocolexchange/protocols/554#/main
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