Team:Harvard/Technology

From 2011.igem.org

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We hope that future iGEM teams will also use these techniques in their own synthetic biology projects.  
We hope that future iGEM teams will also use these techniques in their own synthetic biology projects.  
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=Lambda Red- Mediated Recombineering=
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=Lambda Red Mediated Recombineering=
Genes can be altered by recombination with linear DNA molecules. This requires a high internal DNA concentration, achievable by electroporation. The lambda red system allows efficient recombination between homologous sequences as short as 40 bp, which frees us of the need to provide long tracts of homology for recombination into the chromosome.
Genes can be altered by recombination with linear DNA molecules. This requires a high internal DNA concentration, achievable by electroporation. The lambda red system allows efficient recombination between homologous sequences as short as 40 bp, which frees us of the need to provide long tracts of homology for recombination into the chromosome.
*Gene Knockouts and Exchanges by Linear Transformation: http://rothlab.ucdavis.edu/protocols/Lin.Transform.html
*Gene Knockouts and Exchanges by Linear Transformation: http://rothlab.ucdavis.edu/protocols/Lin.Transform.html

Revision as of 16:24, 15 October 2011

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Overview | MAGE | Chip-Based Synthesis | Lambda Red | Protocols

Our project uses 3 new technologies: Multiplex Automated Genome Engineering (MAGE), chip-based synthesis of DNA, and lamba red recombination, along with more traditional bioinformatics.

Although the project is the first to utilize several key technologies in novel ways, these technologies were developed outside of Harvard iGEM. The multiplex automated genome engineering (MAGE) method was developed by Harris Wang et al in the Church lab of Harvard University. The chip-based synthesis method that we used to synthesize oligos for our zinc finger pool was developed by Sri Kosuri et al in the Church lab, and the actual oligo synthesis was generously provided by Agilent Technologies,a sponsor of iGEM.

We hope that future iGEM teams will also use these techniques in their own synthetic biology projects.

Contents

Bioinformatics

See our Project:Bioinformatics page for details on the computational aspects of our project technology.

Zinc Finger Binding Site Finder

Check out our Zinc Finger Binding Site Finder Tool. This tool was designed and used to search the human genome for the six target DNA sequences that we used to design our custom zinc finger arrays.


Chip-Based Synthesis

Using microchip synthesis (provided by Agilent Technologies), we have 55,000 potential zinc fingers (whose sequences were generated by Team Harvard's bioinformatics) to test. These fingers will then be tried against the DNA sequences we wish to bind.


MAGE

Multiplex automated genome engineering (MAGE) is a new method for large-scale programming and evolution of cells. MAGE simultaneously targets many locations on the chromosome, thus producing combinatorial genomic diversity.


Lambda Red Mediated Recombineering

Genes can be altered by recombination with linear DNA molecules. This requires a high internal DNA concentration, achievable by electroporation. The lambda red system allows efficient recombination between homologous sequences as short as 40 bp, which frees us of the need to provide long tracts of homology for recombination into the chromosome.


Gibson (Isothermal) Assembly

An isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5′ exonuclease, a DNA polymerase and a DNA ligase.

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