Team:Harvard/Results/Lambda Red

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[[Team:Harvard/Results | Overview]] | [[Team:Harvard/Results/MAGE | MAGE]] | [[Team:Harvard/Results/Lambda_Red | Lambda Red]]| [[Team:Harvard/Results/Chip_Synthesis | Chip-Based Library]] | [[Team:Harvard/Results/One-Hybrid_Selection | One-Hybrid Selection]] | [[Team:Harvard/Results/Zinc_Finger_Binders | Zinc Finger Binders]] | [[Team:Harvard/Results/Biobricks | Biobricks]]
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Revision as of 00:43, 26 September 2011

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Overview | Biobricks | Source Code | Acknowledgments | Accomplishments

Fig.1) Gel image illustrating that zeocin has been inserted in place of rpoZ. Colonies 1 through 4 were picked off from a zeocin plate, and were thereby selected for zeocin expression. Lanes 1, 3, 5, 7 show the 650bp band that resulted from doing PCR with rpoZ_R (a primer flanking the rpoZ site) and zeocin_R (a primer internal to the zeocin cassette). Lane 9 has no band, as the control has the endogenous rpoZ and not zeocin at that locus. Lanes 2,4,6 show the larger band when using rpoZ_F and rpoZ_R (the two primers flanking the rpoZ region) to PCR out the zeocin cassette, compared to lane 10, where the same primers are used to PCR out the smaller rpoZ gene. Lane 8 shows no band as the PCR had not worked properly, but lane 7 shows that the zeocin cassette has been inserted into this colony. 915px

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