Team Acknowledgements

Our project was conceived and designed by the Harvard iGEM 2011 advisors and mentors, including but not limited to, Jagesh Shah, Srivatsan Raman, Daniel Goodman, Noah Taylor, Jameson Rogers, Joyce Yang, and Jun Li. The project was made possible by support from the George Church lab at the Wyss Institute for Biologically Inspired Engineering as well as Alain Viel at the Harvard Department of Molecular and Cellular Biology. Without their valuable guidance and great patience, we never would have been able to achieve what have done so far.

Although the project is the first to utilize several key technologies in novel ways, the following technologies were developed outside of iGEM. The multiplex automated genome engineering (MAGE) method was developed by Harris Wang et al in the Church lab. The chip-based synthesis method that we used to synthesize oligos for our zinc finger pool was developed by Sri Kosuri et al in the Church lab, and the actual oligo synthesis was generously provided by Agilent Technologies.

We owe great thanks to Alain Viel for his flawless management of the logistics of iGEM, including but not limited to providing lab space, ordering needed lab supplies, arranging our trip to the Americas Regional, and for his excellent taste in footwear. Furthermore, we would also like to thank our lab manager Andrew Cumming for all of his practical lab advice and for helping to make everything run smoothly in the day to day logistics of lab.

Team Member Acknowledgements

All project work was done by team members, and we would like to make the following acknowledgements:

• Kristin Barclay
  Bioinformatics, chip sequences design and programming, majority of wiki coding and design

• Justin Chew
  Bioinformatics, ZF target selection, ZF expression plasmid construction, Wolfe selection system testing

• William Clerx
  Bioinformatics, ZF target selection, wiki coding and design, video editing, freelance wet lab

• Sarah Choudhury
  TolC selection system construction, TolC and Wolfe selection system testing, biobrick construction

• Naomi Genuth
  Wolfe selection system construction and testing, biobrick construction and registration

• Brandon Gerberich
  Bioinformatics, Wolfe selection system construction and testing, interview cameraman and video editor

• Mark Kopelman
  Wet lab, video editing

• Matt Lunati
  TolC selection system construction and testing

• Nida Naushad
  Bioinformatics, ZF expression plasmid construction, chip library assembly, biobrick construction

Advisor Acknowledgements

Finally, we would like to specially acknowledge all of our teaching fellows (TFs) who have always been there for us through thick and thin, no matter how small or big the problem. Their advice has been of immeasurable value to our project's success. We would also like to also acknowledge the following TFs for their special contributions:

• Daniel Goodman
  Harvard iGEM logo design, wiki design (contributer to CSS and designer of the navigation bar), and being an all around awesome team leader

• Noah Taylor
  Using lambda-red recombination to successfully change our zinc finger binding sites when MAGE was impractical, contributed a reverse translating function from his own research to bioinformatics

• Srivatsan Raman
  Collaborator in our project abstract and Human Practices page