Team:Groningen/project notebook/9 September 2011

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March M T W T F S S   [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/17_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/21_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]	April M T W T F S S         [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_April_2011 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_April_2011 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/17_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_April_2011 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_April_2011 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]	May M T W T F S S             [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_May_2011 2] [http://2011.igem.org/Team:Groningen/project_notebook/3_May_2011 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_May_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_May_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_May_2011 6] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_May_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_May_2011 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_May_2011 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_May_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_May_2011 13] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_May_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_May_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_May_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_May_2011 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_May_2011 20] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/21_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_May_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_May_2011 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_May_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_May_2011 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_May_2011 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/Team:Groningen/project_notebook/30_May_2011 30] [http://2011.igem.org/Team:Groningen/project_notebook/31_May_2011 31]	June M T W T F S S     [http://2011.igem.org/Team:Groningen/project_notebook/1_June_2011 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_June_2011 2] [http://2011.igem.org/Team:Groningen/project_notebook/3_June_2011 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_June_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_June_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_June_2011 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_June_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_June_2011 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_June_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_June_2011 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_June_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_June_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_June_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_June_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_June_2011 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_June_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_June_2011 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_June_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_June_2011 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_June_2011 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_June_2011 28] [http://2011.igem.org/Team:Groningen/project_notebook/29_June_2011 29] [http://2011.igem.org/Team:Groningen/project_notebook/30_June_2011 30]
July M T W T F S S         [http://2011.igem.org/Team:Groningen/project_notebook/1_July_2011 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_July_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_July_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_July_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_July_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_July_2011 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_July_2011 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_July_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_July_2011 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_July_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_July_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_July_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_July_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_July_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_July_2011 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_July_2011 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_July_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_July_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_July_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_July_2011 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_July_2011 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_July_2011 28] [http://2011.igem.org/Team:Groningen/project_notebook/29_July_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]	August M T W T F S S [http://2011.igem.org/Team:Groningen/project_notebook/1_August_2011 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_August_2011 2] [http://2011.igem.org/Team:Groningen/project_notebook/3_August_2011 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_August_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_August_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_August_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_August_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_August_2011 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_August_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_August_2011 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_August_2011 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_August_2011 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_August_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_August_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_August_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_August_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_August_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_August_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_August_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_August_2011 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_August_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_August_2011 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_August_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_August_2011 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/Team:Groningen/project_notebook/29_August_2011 29] [http://2011.igem.org/Team:Groningen/project_notebook/30_August_2011 30] [http://2011.igem.org/Team:Groningen/project_notebook/31_August_2011 31]	September M T W T F S S       [http://2011.igem.org/Team:Groningen/project_notebook/1_September_2011 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_September_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_September_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_September_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_September_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_September_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_September_2011 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_September_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_September_2011 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_September_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_September_2011 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_September_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_September_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_September_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_September_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_September_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_September_2011 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_September_2011 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_September_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_September_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]	October M T W T F S S           [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_October_2011 11] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_October_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_October_2011 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_October_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_October_2011 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_October_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_October_2011 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_October_2011 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_October_2011 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]

Jori
Evaluation strategies

Vessa

To Do List

• Screening 24 variants cotransformants!
• Growth ON culture

Olesja
Flowcytometry measurement of construct: PBad_GFP_DT (colony 4 & 6)
induced with 0.7 % arabinose
measurement: 0-9 h

Joyce

Picked up a new Plasmid DNA isolation kit! :)
Strains for FACS measurements grew!:)
Stickers for sequencing finally arrived!:)

Colony PCR of PBAD-cI-LVA-DT

The conventional taq polymerase tube was empty:( So I borrowed the dreamtaq from MolGen. For more infon about
dreamtaq:http://www.fermentas.com/en/products/all/modifying-enzymes/thermophilic-polymerases/ep070
Addition of MgCl2 in the mastermix will not be necessary, since the Dreamtaq buffer already contains MgCl2.
For the rest, the normal taq mastermix scheme can be used, but the amount of microliters normally used to add MgCl2 need to be

Taq 10× buffer: 40μl
dNTPs 10mM: 8μl
Forward Biobrick primer: 8μl
Reverse Biobrick primer: 8μl
Taq DNA polymerase: 2μl
MQ water: 334μl

PCR conditions:
Preheated lid: 111°C
Denaturation: 94°C for 10 min.
Cycle 33×:
denaturation: 94°C for 30s.
annealing: 60°C for 30s.
Extension: 72°C for 2,5 min.
Final extension: 72°C for 10 min.
Store infinite at 4°C

Analyse on a 1% TBE agarose gel.

Send samples for sequencing!

Inoculated 50μl of the overnight culture in M9+2% casamino acids (preheated)
Let them grow for 1 h
Measure GFP expression with the FACS for PBAD-RBS-GFP-DT colonies 4 and 6

Bought taq DNA polymerase, SpeI, FastAP from the fermentas freezer after receiving the key of it from Sjoerd.

Measurements with the FACS: doing OK!
Colony 6 shows a plot shifting to the right place, showing there is GFP expression after induction with arabinose
Colony 4 also shows a plot shifting to the right place, showing there is GFP expression afterinduction with arabinose, but it
is going slower compared to colony 6...