Team:Groningen/project notebook/31 August 2011

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March M T W T F S S   [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/17_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/21_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]	April M T W T F S S         [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_April_2011 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_April_2011 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/17_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_April_2011 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_April_2011 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]	May M T W T F S S             [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_May_2011 2] [http://2011.igem.org/Team:Groningen/project_notebook/3_May_2011 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_May_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_May_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_May_2011 6] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_May_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_May_2011 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_May_2011 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_May_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_May_2011 13] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_May_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_May_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_May_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_May_2011 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_May_2011 20] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/21_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_May_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_May_2011 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_May_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_May_2011 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_May_2011 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/Team:Groningen/project_notebook/30_May_2011 30] [http://2011.igem.org/Team:Groningen/project_notebook/31_May_2011 31]	June M T W T F S S     [http://2011.igem.org/Team:Groningen/project_notebook/1_June_2011 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_June_2011 2] [http://2011.igem.org/Team:Groningen/project_notebook/3_June_2011 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_June_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_June_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_June_2011 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_June_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_June_2011 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_June_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_June_2011 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_June_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_June_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_June_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_June_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_June_2011 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_June_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_June_2011 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_June_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_June_2011 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_June_2011 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_June_2011 28] [http://2011.igem.org/Team:Groningen/project_notebook/29_June_2011 29] [http://2011.igem.org/Team:Groningen/project_notebook/30_June_2011 30]
July M T W T F S S         [http://2011.igem.org/Team:Groningen/project_notebook/1_July_2011 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_July_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_July_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_July_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_July_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_July_2011 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_July_2011 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_July_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_July_2011 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_July_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_July_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_July_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_July_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_July_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_July_2011 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_July_2011 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_July_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_July_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_July_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_July_2011 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_July_2011 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_July_2011 28] [http://2011.igem.org/Team:Groningen/project_notebook/29_July_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]	August M T W T F S S [http://2011.igem.org/Team:Groningen/project_notebook/1_August_2011 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_August_2011 2] [http://2011.igem.org/Team:Groningen/project_notebook/3_August_2011 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_August_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_August_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_August_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_August_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_August_2011 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_August_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_August_2011 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_August_2011 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_August_2011 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_August_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_August_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_August_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_August_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_August_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_August_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_August_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_August_2011 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_August_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_August_2011 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_August_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_August_2011 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/Team:Groningen/project_notebook/29_August_2011 29] [http://2011.igem.org/Team:Groningen/project_notebook/30_August_2011 30] [http://2011.igem.org/Team:Groningen/project_notebook/31_August_2011 31]	September M T W T F S S       [http://2011.igem.org/Team:Groningen/project_notebook/1_September_2011 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_September_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_September_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_September_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_September_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_September_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_September_2011 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_September_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_September_2011 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_September_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_September_2011 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_September_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_September_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_September_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_September_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_September_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_September_2011 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_September_2011 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_September_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_September_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]	October M T W T F S S           [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_October_2011 11] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_October_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_October_2011 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_October_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_October_2011 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_October_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_October_2011 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_October_2011 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_October_2011 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]

Jori
Presentation vectors / practise

Joyce

Making schemes for this week: Cloning strategy
Today clone cI-LVA and LasR-LVA in pSB1C3-DT, but digest with X and P because the PCR products only contain the XbaI site.
This means that the DT will be excluded. This also means that an extra cloning step will be included to clone the double
terminator in the vector.
Tomorrow: Colony PCR of transformants, also clone PBAD vector in pSB1A3DT.
Friday: Colony PCR of PBAD in pSB1A3DT and if there were right transformants yesterday: plasmid prep, digestion cI-LVA and
LasR-LVA in vector and digest the double terminator PCR product, clean up, ligation, transformation.

So today:
Digestion
pSB1C3DT
3μl vector
1μl XbaI
1μl PstI
3μl FD buffer
1μl FastAP
21μl MQ water

cI-LVA
10μl insert
1μl XbaI
1μl PstI
2μl FD buffer
6μl MQ water

LasR-LVA
11μl
1μl XbaI
1μl PstI
2μl FD buffer
5μl MQ water

Incubate the samples at 37°C for 1h

Clean up the DNA with the High Pure PCR purification kit

Ligation:

cI-LVA-pSB1C
8.5μl vector pSB1C3-DT
8.5μl insert
2μl T4DNA ligase buffer
1μl T4 DNA ligase

LasR-LVA-pSB1C
8.5μl vector pSB1C3-DT
8.5μl insert
2μl T4DNA ligase buffer
1μl T4 DNA ligase

Self ligation pSB1C3-DT
8.5μl vector pSB1C3-DT
8.5μl MQ water
2μl T4DNA ligase buffer
1μl T4 DNA ligase

Incubate samples for 35 min. at room temperature

Transformation
Add to 40μl competent cells 10μl ligation mixture
Incubate for 30 min on ice
Heat shock: 45s at 42 degrees
Incubate cells on ice for 2 min
Add 1ml LB medium+ 25mM glucose
Incubate cells for 1h/1.5h at 37 degrees
Spin the cells down, resuspend them in 100μl LB medium and plate 90μl and 10μl out on the plates
Put the plates in the stove at 37 degrees overnight

At 14:00 the regular meeting will start
At 16:00 the update will start, practicing the presentation and giving comments