Team:Groningen/project notebook/11 July 2011

From 2011.igem.org

March
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	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/17_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 17]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/21_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 21]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]

April
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				[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6]	[http://2011.igem.org/Team:Groningen/project_notebook/7_April_2011 7]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13]	[http://2011.igem.org/Team:Groningen/project_notebook/14_April_2011 14]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/17_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 17]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20]	[http://2011.igem.org/Team:Groningen/project_notebook/21_April_2011 21]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27]	[http://2011.igem.org/Team:Groningen/project_notebook/28_April_2011 28]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]

May
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						[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1]
[http://2011.igem.org/Team:Groningen/project_notebook/2_May_2011 2]	[http://2011.igem.org/Team:Groningen/project_notebook/3_May_2011 3]	[http://2011.igem.org/Team:Groningen/project_notebook/4_May_2011 4]	[http://2011.igem.org/Team:Groningen/project_notebook/5_May_2011 5]	[http://2011.igem.org/Team:Groningen/project_notebook/6_May_2011 6]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8]
[http://2011.igem.org/Team:Groningen/project_notebook/9_May_2011 9]	[http://2011.igem.org/Team:Groningen/project_notebook/10_May_2011 10]	[http://2011.igem.org/Team:Groningen/project_notebook/11_May_2011 11]	[http://2011.igem.org/Team:Groningen/project_notebook/12_May_2011 12]	[http://2011.igem.org/Team:Groningen/project_notebook/13_May_2011 13]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15]
[http://2011.igem.org/Team:Groningen/project_notebook/16_May_2011 16]	[http://2011.igem.org/Team:Groningen/project_notebook/17_May_2011 17]	[http://2011.igem.org/Team:Groningen/project_notebook/18_May_2011 18]	[http://2011.igem.org/Team:Groningen/project_notebook/19_May_2011 19]	[http://2011.igem.org/Team:Groningen/project_notebook/20_May_2011 20]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/21_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 21]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22]
[http://2011.igem.org/Team:Groningen/project_notebook/23_May_2011 23]	[http://2011.igem.org/Team:Groningen/project_notebook/24_May_2011 24]	[http://2011.igem.org/Team:Groningen/project_notebook/25_May_2011 25]	[http://2011.igem.org/Team:Groningen/project_notebook/26_May_2011 26]	[http://2011.igem.org/Team:Groningen/project_notebook/27_May_2011 27]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29]
[http://2011.igem.org/Team:Groningen/project_notebook/30_May_2011 30]	[http://2011.igem.org/Team:Groningen/project_notebook/31_May_2011 31]

June
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		[http://2011.igem.org/Team:Groningen/project_notebook/1_June_2011 1]	[http://2011.igem.org/Team:Groningen/project_notebook/2_June_2011 2]	[http://2011.igem.org/Team:Groningen/project_notebook/3_June_2011 3]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5]
[http://2011.igem.org/Team:Groningen/project_notebook/6_June_2011 6]	[http://2011.igem.org/Team:Groningen/project_notebook/7_June_2011 7]	[http://2011.igem.org/Team:Groningen/project_notebook/8_June_2011 8]	[http://2011.igem.org/Team:Groningen/project_notebook/9_June_2011 9]	[http://2011.igem.org/Team:Groningen/project_notebook/10_June_2011 10]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11]	[http://2011.igem.org/Team:Groningen/project_notebook/12_June_2011 12]
[http://2011.igem.org/Team:Groningen/project_notebook/13_June_2011 13]	[http://2011.igem.org/Team:Groningen/project_notebook/14_June_2011 14]	[http://2011.igem.org/Team:Groningen/project_notebook/15_June_2011 15]	[http://2011.igem.org/Team:Groningen/project_notebook/16_June_2011 16]	[http://2011.igem.org/Team:Groningen/project_notebook/17_June_2011 17]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19]
[http://2011.igem.org/Team:Groningen/project_notebook/20_June_2011 20]	[http://2011.igem.org/Team:Groningen/project_notebook/21_June_2011 21]	[http://2011.igem.org/Team:Groningen/project_notebook/22_June_2011 22]	[http://2011.igem.org/Team:Groningen/project_notebook/23_June_2011 23]	[http://2011.igem.org/Team:Groningen/project_notebook/24_June_2011 24]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26]
[http://2011.igem.org/Team:Groningen/project_notebook/27_June_2011 27]	[http://2011.igem.org/Team:Groningen/project_notebook/28_June_2011 28]	[http://2011.igem.org/Team:Groningen/project_notebook/29_June_2011 29]	[http://2011.igem.org/Team:Groningen/project_notebook/30_June_2011 30]

July
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				[http://2011.igem.org/Team:Groningen/project_notebook/1_July_2011 1]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3]
[http://2011.igem.org/Team:Groningen/project_notebook/4_July_2011 4]	[http://2011.igem.org/Team:Groningen/project_notebook/5_July_2011 5]	[http://2011.igem.org/Team:Groningen/project_notebook/6_July_2011 6]	[http://2011.igem.org/Team:Groningen/project_notebook/7_July_2011 7]	[http://2011.igem.org/Team:Groningen/project_notebook/8_July_2011 8]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10]
[http://2011.igem.org/Team:Groningen/project_notebook/11_July_2011 11]	[http://2011.igem.org/Team:Groningen/project_notebook/12_July_2011 12]	[http://2011.igem.org/Team:Groningen/project_notebook/13_July_2011 13]	[http://2011.igem.org/Team:Groningen/project_notebook/14_July_2011 14]	[http://2011.igem.org/Team:Groningen/project_notebook/15_July_2011 15]	[http://2011.igem.org/Team:Groningen/project_notebook/16_July_2011 16]	[http://2011.igem.org/Team:Groningen/project_notebook/17_July_2011 17]
[http://2011.igem.org/Team:Groningen/project_notebook/18_July_2011 18]	[http://2011.igem.org/Team:Groningen/project_notebook/19_July_2011 19]	[http://2011.igem.org/Team:Groningen/project_notebook/20_July_2011 20]	[http://2011.igem.org/Team:Groningen/project_notebook/21_July_2011 21]	[http://2011.igem.org/Team:Groningen/project_notebook/22_July_2011 22]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24]
[http://2011.igem.org/Team:Groningen/project_notebook/25_July_2011 25]	[http://2011.igem.org/Team:Groningen/project_notebook/26_July_2011 26]	[http://2011.igem.org/Team:Groningen/project_notebook/27_July_2011 27]	[http://2011.igem.org/Team:Groningen/project_notebook/28_July_2011 28]	[http://2011.igem.org/Team:Groningen/project_notebook/29_July_2011 29]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]

August
M	T	W	T	F	S	S
[http://2011.igem.org/Team:Groningen/project_notebook/1_August_2011 1]	[http://2011.igem.org/Team:Groningen/project_notebook/2_August_2011 2]	[http://2011.igem.org/Team:Groningen/project_notebook/3_August_2011 3]	[http://2011.igem.org/Team:Groningen/project_notebook/4_August_2011 4]	[http://2011.igem.org/Team:Groningen/project_notebook/5_August_2011 5]	[http://2011.igem.org/Team:Groningen/project_notebook/6_August_2011 6]	[http://2011.igem.org/Team:Groningen/project_notebook/7_August_2011 7]
[http://2011.igem.org/Team:Groningen/project_notebook/8_August_2011 8]	[http://2011.igem.org/Team:Groningen/project_notebook/9_August_2011 9]	[http://2011.igem.org/Team:Groningen/project_notebook/10_August_2011 10]	[http://2011.igem.org/Team:Groningen/project_notebook/11_August_2011 11]	[http://2011.igem.org/Team:Groningen/project_notebook/12_August_2011 12]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13]	[http://2011.igem.org/Team:Groningen/project_notebook/14_August_2011 14]
[http://2011.igem.org/Team:Groningen/project_notebook/15_August_2011 15]	[http://2011.igem.org/Team:Groningen/project_notebook/16_August_2011 16]	[http://2011.igem.org/Team:Groningen/project_notebook/17_August_2011 17]	[http://2011.igem.org/Team:Groningen/project_notebook/18_August_2011 18]	[http://2011.igem.org/Team:Groningen/project_notebook/19_August_2011 19]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20]	[http://2011.igem.org/Team:Groningen/project_notebook/21_August_2011 21]
[http://2011.igem.org/Team:Groningen/project_notebook/22_August_2011 22]	[http://2011.igem.org/Team:Groningen/project_notebook/23_August_2011 23]	[http://2011.igem.org/Team:Groningen/project_notebook/24_August_2011 24]	[http://2011.igem.org/Team:Groningen/project_notebook/25_August_2011 25]	[http://2011.igem.org/Team:Groningen/project_notebook/26_August_2011 26]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28]
[http://2011.igem.org/Team:Groningen/project_notebook/29_August_2011 29]	[http://2011.igem.org/Team:Groningen/project_notebook/30_August_2011 30]	[http://2011.igem.org/Team:Groningen/project_notebook/31_August_2011 31]

September
M	T	W	T	F	S	S
			[http://2011.igem.org/Team:Groningen/project_notebook/1_September_2011 1]	[http://2011.igem.org/Team:Groningen/project_notebook/2_September_2011 2]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3]	[http://2011.igem.org/Team:Groningen/project_notebook/4_September_2011 4]
[http://2011.igem.org/Team:Groningen/project_notebook/5_September_2011 5]	[http://2011.igem.org/Team:Groningen/project_notebook/6_September_2011 6]	[http://2011.igem.org/Team:Groningen/project_notebook/7_September_2011 7]	[http://2011.igem.org/Team:Groningen/project_notebook/8_September_2011 8]	[http://2011.igem.org/Team:Groningen/project_notebook/9_September_2011 9]	[http://2011.igem.org/Team:Groningen/project_notebook/10_September_2011 10]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11]
[http://2011.igem.org/Team:Groningen/project_notebook/12_September_2011 12]	[http://2011.igem.org/Team:Groningen/project_notebook/13_September_2011 13]	[http://2011.igem.org/Team:Groningen/project_notebook/14_September_2011 14]	[http://2011.igem.org/Team:Groningen/project_notebook/15_September_2011 15]	[http://2011.igem.org/Team:Groningen/project_notebook/16_September_2011 16]	[http://2011.igem.org/Team:Groningen/project_notebook/17_September_2011 17]	[http://2011.igem.org/Team:Groningen/project_notebook/18_September_2011 18]
[http://2011.igem.org/Team:Groningen/project_notebook/19_September_2011 19]	[http://2011.igem.org/Team:Groningen/project_notebook/20_September_2011 20]	[http://2011.igem.org/Team:Groningen/project_notebook/21_September_2011 21]	[http://2011.igem.org/Team:Groningen/project_notebook/22_September_2011 22]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]

October
M	T	W	T	F	S	S
					[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10]	[http://2011.igem.org/Team:Groningen/project_notebook/11_October_2011 11]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16]
[http://2011.igem.org/Team:Groningen/project_notebook/17_October_2011 17]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20]	[http://2011.igem.org/Team:Groningen/project_notebook/21_October_2011 21]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22]	[http://2011.igem.org/Team:Groningen/project_notebook/23_October_2011 23]
[http://2011.igem.org/Team:Groningen/project_notebook/24_October_2011 24]	[http://2011.igem.org/Team:Groningen/project_notebook/25_October_2011 25]	[http://2011.igem.org/Team:Groningen/project_notebook/26_October_2011 26]	[http://2011.igem.org/Team:Groningen/project_notebook/27_October_2011 27]	[http://2011.igem.org/Team:Groningen/project_notebook/28_October_2011 28]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29]	[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]
[http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]

Joyce
PCR clean up of the pBAD araC PCR products.
Digestion of pBAD araC, cI-LVA, LasR-LVA and the vectors pSB1A3+DT and pSB1A3 + RBS-GFP
Digestion
pBAD araC:
pBADaraC: 10μl
EcoRI: 1μl
SpeI: 1μl
Fast digest buffer: 2μl
MilliQ water: 6μl

cI-LVA:
cI-LVA: 2μl
EcoRI: 1μl
SpeI: 1μl
Fast digest buffer: 2μl
MilliQ water: 14μl

LasR-LVA:
LasR-LVA: 3μl
EcoRI: 1μl
SpeI: 1μl
Fast digest buffer: 2μl
MilliQ water: 13μl

pSB1A3+DT:
pSB1A3+DT: 3μl
EcoRI: 1μl
SpeI: 1μl --> XbaI?
Fast digest buffer: 2μl
Fast alkaline phosphatase: 1μl
MilliQ water: 22μl

pSB1A3+RBS-GFP:
pSB1A3+RBS-GFP: 3μl
EcoRI: 1μl
SpeI: 1μl --> XbaI?
Fast digest buffer: 2μl
Fast alkaline phosphatase: 1μl
MilliQ water: 22μl

Incubate at 37 °C for 30 minutes
Do after the digestion a DNA clean up with the High Pure PCR purification kit --> how much MQ did you use for DNA elution?
Ligation
Calculate with the ligation calculator how much ng DNA is needed for your ligation and then calculate how many microliters this is.
cI-LVA in pSB1A3-DT
cI-LVA: 7.8 μl
pSB1A3-DT: 8.5μl
T4 DNA ligase buffer: 2μl
T4 DNA ligase: 1μl
MilliQ water: 0.7μl

LasR-LVA in pSB1A3-DT
LasR-LVA: 6.1 μl
pSB1A3-DT: 8.5μl
T4 DNA ligase buffer: 2μl
T4 DNA ligase: 1μl
MilliQ water: 2.4μl

pBAD araC in pSB1A3+RBS-GFP
pBAD araC: 7.8 μl
pSB1A3+ RBS-GFP: 8.5μl
T4 DNA ligase buffer: 2μl
T4 DNA ligase: 1μl
MilliQ water: 0.7μl

pSB1A3 self ligation control
pSB1A3-DT: 8.5μl
T4 DNA ligase buffer: 2μl
T4 DNA ligase: 1μl
MilliQ water: 8.5μl

pSB1A3+RBS-GFP
pSB1A3+RBS-GFP: 8.5μl
T4 DNA ligase buffer: 2μl
T4 DNA ligase: 1μl
MilliQ water: 8.5μl

Incubate for 30 minutes at roomtemperature.
Transformation:
- Add to 40μl competent cells 10μl DNA ligation mixture
- Incubate 30 minutes on ice
- Do the heatshock: incubate 1min at 42°C
- Place the tubes on ice for 2 minutes and then pipette 1 ml of LB medium + 25mM glucose
- Incubate the tubes for 1 hour (with maximum of 1.5h) at 37°C
- Pellet the cells and resuspend them in 100μl medium
- Plate 90μl and 10μl of the cells on LB+ampicillin plates
- Incubate overnight at 37°C

Jakub
Week 28:

Cloning LasR variants into plasmids with reverse double terminator and LasB promoter - second try.

Christoph

Cloning rev.PLasB+DT into pSB1K3+revDT+PcI+cI