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Team:Groningen/project AND gate
From 2011.igem.org
AND gate
In order to integrate first and second memory unit, we designed the AND gate system. In the second occurrence, the first memory unit runs, the AND gate activates the second memory unit. To modulate gene expression at the translation level, we harness the use of engineered RNA-based/riboregulator system. After transcription, the 5’ UTR of crRNA-LasR transcript will form a stem-loop structure that block RBS and prevents translation. To activate the expression of self-repressed gene, taRNA which is complementary to crRNA will unfold the stem-loop structure thus exposing the obstructed RBS and permit translation. (Isaacs, 2006)
The designed of AND gate circuit based on two constructs, noncoding taRNA-DT cloned downstream of a hybB promoter (PhybB) and a cI promoter (PcI) cloned upstream crRNA-RBS-LasR-TAG-DT. In attempt to characterize the functional assembled parts, we cotransformed plasmid harboring AND gate system with reporter plasmid downstream LasR promoter.
