The RsmA system has a homologous in Escherichia Coli named CsrA. We know these two system are extremely closed on structural and functional sides. The most difference between this two regulation systems is on target regulon. For example, in Pseudomonas aeruginosa Rsma regulate many virulence genes as type III secretion system (anja Brencic and Stephen Lory). In Escherichia coli, RsmA homologous regulate metabolic network. Our goal is to integrate this translational regulation system in the toggle switch. We need to know whether it influence the bacteria’s life.

Figure 1 present growth curve of DH5α carries into a plasmid pVLT31 with or without rsmA and Natural RBS cloned downstream the Plac promoter. Two triads can be seen. The triad containing the strains with empty plasmid shows an upper growth curve compared to the second triad carries pVLT31-rsmA. But it’s important to say that the two groups start their growth not at the same value. That explains a time lag between these two groups. After normalization of all curves, no differences between these two kind of bacteria could be seen. We conclude that the RsmA overexpression hasn’t effects on the growth of bacteria.