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Team:Grenoble/Projet/Modelling/Parameters
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Revision as of 18:46, 23 October 2011
Modelling - Parameters
Here you can find our set of parameters and the study of the sensitivity to parameters of our system.Parameters
PDF file (detailed explanation on parameters and how we deduced them) for Toggle Switch
| kplac | pLac production rate | 600 proteins/min | [1] |
| kpTet | pTet production rate | 600 proteins/min | [1] |
| kpMer | pMer production rate | 600 proteins/min | [1] |
| δLacI | LacI degradation rate | 4.6E-2 min−1 | PDF file |
| δTetR | TetR degradation rate | 4.6E-2 min−1 | PDF file |
| δMerR | MerR degradation rate | 4.6E-2 min−1 | PDF file |
| KpLac - LacI | plac - LacI dissociation constant | 5.45E-7 M | Kyoto 10 |
| KpMer - MerR | pMer - MerR dissociation constant | 1.00E-8 M | [2] |
| KpTet - TerR | pTet - TetR dissociation constant | 5.00E-8 M | [3] |
| KLacI - IPTG | LacI - IPTG dissociation constant | 2.96E-5 M | [4] |
| KTetR - aTc | aTc - TetR dissociation constant | 1.5E-8 M | [5] |
| KHg2+ - MerR | Hg2+ - MerR dissociation constant | 1E-7 M | [2] |
| nplac | plac cooperativity number | 2,3 | [4] |
| npMer | pMer cooperativity number | 2,5 | [6] |
| npTet | pTet cooperativity number | 3 | [7] |
Sensitivity to parameters
References
[1] Nature. 2000 Jan 20;403(6767):335-8. A synthetic oscillatory network of transcriptional regulators. Elowitz MB, Leibler S.
[2] Mol Gen Genet. 1999 Aug;262(1):154-62. Purification and characterization of MerR, the regulator of the broad-spectrum mercury resistance genes in Streptomyces lividans 1326. Rother D, Mattes R, Altenbuchner J.
[3] David Braun et al. Parameter estimation for two synthetic gene networks: A case study. IEEE 2005.
[4] Nature 403, 339-342 (20 January 2000) Construction of a genetic toggle switch in Escherichia coli. Timothy S. Gardner Charles R. Cantor & James J. Collins
[5] O. Scholz, P. Schubert, M. Kintrup and W. Hillen, Tet repressor induction without Mg2+, Biochemistry 39 (2000), pp. 10914–10920
[6] Ultrasensitivity and heavy-metal selectivity of the allosterically modulated MerR transcription complex. D M Ralston and T V O'Halloran
[7] Systems analysis of a quorum sensing network: design constraints imposed by the functional requirements, network topology and kinetic constants. Goryachev AB, Toh DJ, Lee T.
