Team:ETH Zurich/Achievements/Data page

From 2011.igem.org

(Difference between revisions)
(Characterized New Parts)
(Our Favorite New Parts)
 
Line 27: Line 27:
#[http://partsregistry.org/Part:BBa_K625000 MainPage]-'''LacI<sub>M1</sub>''', BBa_K625000; ''E. coli'' codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI<sub>M1</sub> binds to P<sub>lac</sub> and inhibits transcription. ([[Team:ETH_Zurich/Biology/Validation#Bandpass-Characterization_of_lacIM1|Characterization]])  
#[http://partsregistry.org/Part:BBa_K625000 MainPage]-'''LacI<sub>M1</sub>''', BBa_K625000; ''E. coli'' codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI<sub>M1</sub> binds to P<sub>lac</sub> and inhibits transcription. ([[Team:ETH_Zurich/Biology/Validation#Bandpass-Characterization_of_lacIM1|Characterization]])  
#[http://partsregistry.org/Part:BBa_K625005 MainPage]-'''Psb6A5''', BBa_K625005; improved Plasmid [http://partsregistry.org/Part:pSB6A1 pSB6A1] (ETHZ, 2007): transcriptional terminators added. ([[Team:ETH_Zurich/Biology/Validation#Copy_number_test|Characterization]])
#[http://partsregistry.org/Part:BBa_K625005 MainPage]-'''Psb6A5''', BBa_K625005; improved Plasmid [http://partsregistry.org/Part:pSB6A1 pSB6A1] (ETHZ, 2007): transcriptional terminators added. ([[Team:ETH_Zurich/Biology/Validation#Copy_number_test|Characterization]])
-
#[http://partsregistry.org/Part:BBa_K625003 MainPage]-'''P<sub>U</sub> promoter''' BBa_K625003; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow 2007) Promoter regulated by the transcriptional activator XylR (shortened version) ([[Team:ETH_Zurich/Biology/Validation#XylR_-_the_xylene_sensor|Characterization]] and [[Team:ETH_Zurich/Biology/Validation#Dose_response_curve|Dose response fit]])
+
#[http://partsregistry.org/Part:BBa_K625003 MainPage]-'''P<sub>U</sub> promoter''' BBa_K625003; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007) Promoter regulated by the transcriptional activator XylR (shortened version) ([[Team:ETH_Zurich/Biology/Validation#XylR_-_the_xylene_sensor|Characterization]] and [[Team:ETH_Zurich/Biology/Validation#Dose_response_curve|Dose response fit]])
{{:Team:ETH Zurich/Templates/SectionEnd}}
{{:Team:ETH Zurich/Templates/SectionEnd}}
{{:Team:ETH Zurich/Templates/SectionStart}}
{{:Team:ETH Zurich/Templates/SectionStart}}

Latest revision as of 23:59, 28 October 2011

Can you feel the smoke tonight?
 

Contents

SmoColi-Data page

SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.

How Our System Works


Our Favorite New Parts

  1. MainPage-LacIM1, BBa_K625000; E. coli codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacIM1 binds to Plac and inhibits transcription. (Characterization)
  2. MainPage-Psb6A5, BBa_K625005; improved Plasmid pSB6A1 (ETHZ, 2007): transcriptional terminators added. (Characterization)
  3. MainPage-PU promoter BBa_K625003; improved version of BBa_I723138 (Glasgow, 2007) Promoter regulated by the transcriptional activator XylR (shortened version) (Characterization and Dose response fit)


Characterized New Parts

  1. MainPage- PTet - LacIM1 - Termination, BBa_K625001 (Characterization)
  2. MainPage-PU promoter, BBa_K625002; improved version of BBa_I723138 (Glasgow, 2007); Promoter regulated by the transcriptional activator XylR (long version with stop codon) (Characterization)


Characterized Pre-existing Parts

  1. Experience-PBAD promoter, BBa_I13453 (Endy Lab, 2005) dose response for homogenous expression of the PBAD in BW27783 (Characterization)


Back to iGEM Our Sponsors
ETHZ-BASF.png ETH Zurich Logo.png ETHZ-Lonza.png ETHZ-Merck Serono.png
ETHZ-Novartis.png ETHZ-Roche.png ETHZ-Syngenta.png DSM MasterLogo.png