Team:ETH Zurich/Achievements/Data page

From 2011.igem.org

(Difference between revisions)
(→Data For Our Favorite New Parts)
(→Our Favorite New Parts)
 
(24 intermediate revisions not shown)
Line 2: Line 2:
{{:Team:ETH Zurich/Templates/SectionStart}}
{{:Team:ETH Zurich/Templates/SectionStart}}
-
= Data page =
+
= SmoColi-Data page =
-
'''SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.'''
+
'''SmoColi cells are engineered to sense toxic substances found in cigarette smoke.''' They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.
{{:Team:ETH Zurich/Templates/SectionEnd}}
{{:Team:ETH Zurich/Templates/SectionEnd}}
{{:Team:ETH Zurich/Templates/SectionStart}}
{{:Team:ETH Zurich/Templates/SectionStart}}
-
== How Our System Works ==
+
 
 +
= How Our System Works =
<center>
<center>
<html>
<html>
<img src="/wiki/images/a/ac/ETHZ_Data_page.png" usemap="#Datapage1"/>
<img src="/wiki/images/a/ac/ETHZ_Data_page.png" usemap="#Datapage1"/>
<map name="Datapage1">
<map name="Datapage1">
-
<area shape="rect" alt="" title="" coords="278,135,433,183" href="#Data_For_Our_Favorite_New_Parts"/>
+
<area shape="rect" alt="" title="" coords="217,122,433,164" href="#Our_Favorite_New_Parts"/>
-
<area shape="rect" alt="" title="" coords="279,184,433,201" href="#Characterized_Existing_Parts"/>
+
<area shape="rect" alt="" title="" coords="217,169,433,186" href="#Characterized New Parts "/>
-
<area shape="rect" alt="" title="" coords="6,292,794,330" href="#Data_For_Our_Favorite_New_Parts"/>
+
<area shape="rect" alt="" title="" coords="0,260,716,296" href="#Our_Favorite_New_Parts"/>
-
<area shape="rect" alt="" title="" coords="510,106,583,144" href="#Data_For_Our_Favorite_New_Parts"/>
+
<area shape="rect" alt="" title="" coords="464,94,532,130" href="#Our_Favorite_New_Parts"/>
 +
<area shape="rect" alt="" title="" coords="216,192,434,252" href="#Characterized_Pre-existing_Parts"/>
</map>
</map>
</html>
</html>
Line 22: Line 24:
{{:Team:ETH Zurich/Templates/SectionStart}}
{{:Team:ETH Zurich/Templates/SectionStart}}
-
== Data For Our Favorite New Parts ==
+
= Our Favorite New Parts =
-
#[http://partsregistry.org/Part:BBa_K625000 MainPage]-'''LacI<sub>M1</sub>''', BBa_K625000; ''E. coli'' codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI<sub>M1</sub> binds to P<sub>lac</sub> and inhibits transcription. [[Team:ETH_Zurich/Biology/Validation#Bandpass-Characterization_of_lacIM1|Characterization]]  
+
#[http://partsregistry.org/Part:BBa_K625000 MainPage]-'''LacI<sub>M1</sub>''', BBa_K625000; ''E. coli'' codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacI<sub>M1</sub> binds to P<sub>lac</sub> and inhibits transcription. ([[Team:ETH_Zurich/Biology/Validation#Bandpass-Characterization_of_lacIM1|Characterization]])
-
#[http://partsregistry.org/Part:BBa_K625005 MainPage]-'''Psb6A5''', BBa_K625005; improved Plasmid pSB6A1 (ETHZ, 2007): transcriptional terminators added. ([[Team:ETH_Zurich/Biology/Validation#Copy_number_test|Characterization]])
+
#[http://partsregistry.org/Part:BBa_K625005 MainPage]-'''Psb6A5''', BBa_K625005; improved Plasmid [http://partsregistry.org/Part:pSB6A1 pSB6A1] (ETHZ, 2007): transcriptional terminators added. ([[Team:ETH_Zurich/Biology/Validation#Copy_number_test|Characterization]])
-
#[http://partsregistry.org/Part:BBa_K625003 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625003; Promoter regulated by the transcriptional activator XylR (shortened version) ([[https://2011.igem.org/Team:ETH_Zurich/Biology/Validation#XylR_-_the_xylene_sensor Characterization]])
+
#[http://partsregistry.org/Part:BBa_K625003 MainPage]-'''P<sub>U</sub> promoter''' BBa_K625003; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007) Promoter regulated by the transcriptional activator XylR (shortened version) ([[Team:ETH_Zurich/Biology/Validation#XylR_-_the_xylene_sensor|Characterization]] and [[Team:ETH_Zurich/Biology/Validation#Dose_response_curve|Dose response fit]])
 +
{{:Team:ETH Zurich/Templates/SectionEnd}}
 +
{{:Team:ETH Zurich/Templates/SectionStart}}
 +
 
 +
=Characterized New Parts =
 +
#[http://partsregistry.org/Part:BBa_K625001 MainPage]-''' P<sub>Tet</sub> - LacI<sub>M1</sub> - Termination''', BBa_K625001 ([[Team:ETH_Zurich/Biology/Validation#Bandpass-Characterization_of_lacIM1|Characterization]])
 +
#[http://partsregistry.org/Part:BBa_K625002 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625002; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007); Promoter regulated by the transcriptional activator XylR (long version with stop codon) ([[Team:ETH_Zurich/Biology/Validation#XylR_-_the_xylene_sensor|Characterization]])
{{:Team:ETH Zurich/Templates/SectionEnd}}
{{:Team:ETH Zurich/Templates/SectionEnd}}
{{:Team:ETH Zurich/Templates/SectionStart}}
{{:Team:ETH Zurich/Templates/SectionStart}}
-
= Characterized Existing Parts =
+
=Characterized Pre-existing Parts =
-
#[http://partsregistry.org/Part:BBa_K625001 MainPage]-''' P<sub>Tet</sub> - LacI<sub>M1</sub> - Termination''', BBa_K625001 ([[Team:ETH_Zurich/Biology/Validation#Characterization_of_lacIM1|Characterization]])
+
#[http://partsregistry.org/Part:BBa_I13453:Experience Experience]-'''P<sub>BAD</sub> promoter''', BBa_I13453 (Endy Lab, 2005) dose response for homogenous expression of the P<sub>BAD</sub> in BW27783 ([[Team:ETH_Zurich/Biology/Validation#AraC_-_the_arabinose_sensor|Characterization]])
-
#[http://partsregistry.org/Part:BBa_K625002 MainPage]-'''P<sub>U</sub> promoter''', BBa_K625002; Promoter regulated by the transcriptional activator XylR (long version with stop codon) ([[Team:ETH_Zurich/Biology/Validation#XylR- the xylene sensor|Characterization]])
+
{{:Team:ETH Zurich/Templates/SectionEnd}}
{{:Team:ETH Zurich/Templates/SectionEnd}}
{{:Team:ETH Zurich/Templates/HeaderNewEnd}}
{{:Team:ETH Zurich/Templates/HeaderNewEnd}}

Latest revision as of 23:59, 28 October 2011

Can you feel the smoke tonight?
 

Contents

SmoColi-Data page

SmoColi cells are engineered to sense toxic substances found in cigarette smoke. They are immobilized in a microfluidic channel, in which a concentration gradient of the toxic substance is established. The sensor is linked to a band-pass filter that leads to input-concentration-dependent GFP expression. Continuous increase of the input concentration and its detection, therefore, establishes a moving fluorescent band in the channel. Finally, if the input concentration exceeds a certain threshold, cells produce RFP and the device turns red.

How Our System Works


Our Favorite New Parts

  1. [http://partsregistry.org/Part:BBa_K625000 MainPage]-LacIM1, BBa_K625000; E. coli codon-modified LacI designed to avoid recombination with the wild type LacI in a system where both variants are present, LacIM1 binds to Plac and inhibits transcription. (Characterization)
  2. [http://partsregistry.org/Part:BBa_K625005 MainPage]-Psb6A5, BBa_K625005; improved Plasmid [http://partsregistry.org/Part:pSB6A1 pSB6A1] (ETHZ, 2007): transcriptional terminators added. (Characterization)
  3. [http://partsregistry.org/Part:BBa_K625003 MainPage]-PU promoter BBa_K625003; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007) Promoter regulated by the transcriptional activator XylR (shortened version) (Characterization and Dose response fit)


Characterized New Parts

  1. [http://partsregistry.org/Part:BBa_K625001 MainPage]- PTet - LacIM1 - Termination, BBa_K625001 (Characterization)
  2. [http://partsregistry.org/Part:BBa_K625002 MainPage]-PU promoter, BBa_K625002; improved version of [http://partsregistry.org/Part:BBa_I723138 BBa_I723138] (Glasgow, 2007); Promoter regulated by the transcriptional activator XylR (long version with stop codon) (Characterization)


Characterized Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_I13453:Experience Experience]-PBAD promoter, BBa_I13453 (Endy Lab, 2005) dose response for homogenous expression of the PBAD in BW27783 (Characterization)


Back to iGEM Our Sponsors
ETHZ-BASF.png ETH Zurich Logo.png ETHZ-Lonza.png ETHZ-Merck Serono.png
ETHZ-Novartis.png ETHZ-Roche.png ETHZ-Syngenta.png DSM MasterLogo.png
 

Retrieved from "http://2011.igem.org/Team:ETH_Zurich/Achievements/Data_page"